Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GA721_RS11510 Genome accession   NZ_CP045131
Coordinates   2430957..2431754 (-) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain TQ18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2425957..2436754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA721_RS11490 - 2427000..2427704 (-) 705 WP_005182401.1 YfgM family protein -
  GA721_RS11495 hisS 2427735..2429030 (-) 1296 WP_104472417.1 histidine--tRNA ligase -
  GA721_RS11500 ispG 2429034..2430155 (-) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GA721_RS11505 - 2430178..2430966 (-) 789 WP_016659532.1 helix-turn-helix domain-containing protein -
  GA721_RS11510 pilF 2430957..2431754 (-) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GA721_RS11515 rlmN 2431790..2433025 (-) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GA721_RS11520 ndk 2433148..2433579 (-) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  GA721_RS11525 iscX 2433689..2433889 (-) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  GA721_RS11530 - 2433984..2434535 (-) 552 WP_104470590.1 phosphatase PAP2 family protein -
  GA721_RS11535 - 2434568..2435851 (-) 1284 WP_160243527.1 glycosyltransferase family 4 protein -
  GA721_RS11540 - 2436019..2436438 (-) 420 WP_005182377.1 hypothetical protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=392181 GA721_RS11510 WP_104470591.1 2430957..2431754(-) (pilF) [Acinetobacter indicus strain TQ18]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=392181 GA721_RS11510 WP_104470591.1 2430957..2431754(-) (pilF) [Acinetobacter indicus strain TQ18]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCGCTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGCAGTAAGCTGAATCTGGAGAAGGCCGATACGTATTTCAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCTCTGCAGGCGAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCCGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTAGGACAAAAAAATCAAGGTG
CGCGTGCCTTATGGATTGGGATCCGTGTTGCACGTGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment