Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GA646_RS12325 Genome accession   NZ_CP045127
Coordinates   2412688..2413488 (-) Length   266 a.a.
NCBI ID   WP_104489142.1    Uniprot ID   -
Organism   Acinetobacter indicus strain XG03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2407688..2418488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA646_RS12305 - 2408731..2409435 (-) 705 WP_104489140.1 YfgM family protein -
  GA646_RS12310 hisS 2409466..2410761 (-) 1296 WP_104470593.1 histidine--tRNA ligase -
  GA646_RS12315 ispG 2410765..2411886 (-) 1122 WP_104489141.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GA646_RS12320 - 2411909..2412697 (-) 789 WP_075175118.1 helix-turn-helix domain-containing protein -
  GA646_RS12325 pilF 2412688..2413488 (-) 801 WP_104489142.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GA646_RS12330 rlmN 2413509..2414744 (-) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GA646_RS12335 ndk 2414867..2415298 (-) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  GA646_RS12340 iscX 2415408..2415608 (-) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  GA646_RS12345 - 2415703..2416254 (-) 552 WP_005182381.1 phosphatase PAP2 family protein -
  GA646_RS12350 - 2416287..2417570 (-) 1284 WP_104489143.1 glycosyltransferase family 4 protein -
  GA646_RS12355 - 2417738..2418157 (-) 420 WP_005182377.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30181.36 Da        Isoelectric Point: 9.2581

>NTDB_id=392098 GA646_RS12325 WP_104489142.1 2412688..2413488(-) (pilF) [Acinetobacter indicus strain XG03]
MRKPSLKFICTSILGLTLVVSGGCQTTGAKGDPEKAVKVRTQLAAEYIKVNNFDAAKRALDQALEINSRDATANMMMGVL
LQQEGSKFNVERAERYFKQALSIEPKNAQARNNYGTYLYQVGRYNDAIEHLQIAGTTLGYDQRFRALENLGRAYVQLGKL
KEAEQAFIQALQINRDAYIAMLELAELKYFNQQQAMATELYEDFVRGVGQNNQDARALWIGIRIARANQDNLGTQVLVNQ
LRALYPESQEYQRYLQLKYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=392098 GA646_RS12325 WP_104489142.1 2412688..2413488(-) (pilF) [Acinetobacter indicus strain XG03]
TTGAGAAAGCCAAGCTTGAAATTTATTTGCACTTCTATTTTGGGGCTGACCTTAGTTGTTTCTGGTGGATGTCAGACTAC
CGGTGCCAAAGGTGATCCAGAAAAAGCAGTAAAGGTAAGAACTCAACTGGCAGCTGAATACATTAAAGTCAATAACTTTG
ATGCAGCGAAACGTGCACTTGATCAGGCACTTGAGATTAATTCTCGCGATGCAACAGCCAATATGATGATGGGCGTGCTG
TTACAGCAAGAAGGAAGCAAGTTTAATGTAGAGCGAGCAGAGCGCTATTTTAAACAGGCACTTTCTATTGAGCCAAAAAA
TGCGCAGGCACGTAACAACTATGGTACGTATTTGTATCAGGTGGGGCGTTATAATGATGCGATTGAACATCTCCAAATAG
CAGGAACAACTTTGGGATATGATCAACGTTTTCGTGCATTGGAAAATTTAGGTCGAGCTTATGTGCAACTGGGTAAATTA
AAAGAGGCGGAACAAGCTTTTATTCAGGCACTACAGATTAATCGTGATGCCTATATTGCTATGCTTGAATTGGCTGAGTT
GAAGTATTTCAATCAGCAACAAGCAATGGCGACAGAACTTTATGAAGATTTTGTACGCGGTGTCGGGCAAAATAATCAAG
ATGCGCGTGCTTTATGGATCGGGATTCGTATTGCTCGAGCAAACCAAGACAATTTGGGGACACAGGTGTTGGTCAATCAA
CTGCGTGCCTTGTATCCAGAGAGTCAGGAATACCAACGTTATCTGCAATTGAAATACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(89-107)

Tetratricopeptide repeat (TPR_16)

(43-81)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.165

100

0.684
  pilF Acinetobacter baylyi ADP1

63.813

96.617

0.617

Multiple sequence alignment