Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GA645_RS05500 Genome accession   NZ_CP045118
Coordinates   1183332..1184834 (-) Length   500 a.a.
NCBI ID   WP_152220667.1    Uniprot ID   -
Organism   Pseudomonas sp. SCB32     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1178332..1189834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA645_RS05470 yjgA 1179253..1179777 (-) 525 WP_256676074.1 ribosome biogenesis factor YjgA -
  GA645_RS05475 pmbA 1179896..1181245 (+) 1350 WP_152220660.1 metalloprotease PmbA -
  GA645_RS05480 - 1181306..1181578 (-) 273 WP_152220662.1 HPr family phosphocarrier protein -
  GA645_RS05485 rapZ 1181611..1182468 (-) 858 WP_152220664.1 RNase adapter RapZ -
  GA645_RS05490 ptsN 1182471..1182935 (-) 465 WP_152220665.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GA645_RS05495 hpf 1182945..1183253 (-) 309 WP_017518151.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GA645_RS05500 rpoN/rpoN1 1183332..1184834 (-) 1503 WP_152220667.1 RNA polymerase factor sigma-54 Machinery gene
  GA645_RS05505 lptB 1185076..1185801 (-) 726 WP_152220669.1 LPS export ABC transporter ATP-binding protein -
  GA645_RS05510 lptA 1185801..1186337 (-) 537 WP_152220671.1 lipopolysaccharide transport periplasmic protein LptA -
  GA645_RS05515 lptC 1186324..1186896 (-) 573 WP_152220673.1 LPS export ABC transporter periplasmic protein LptC -
  GA645_RS05520 - 1186905..1187429 (-) 525 WP_152220675.1 HAD family hydrolase -
  GA645_RS05525 - 1187430..1188404 (-) 975 WP_152220678.1 KpsF/GutQ family sugar-phosphate isomerase -
  GA645_RS05530 - 1188646..1189455 (+) 810 WP_152220680.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 56040.03 Da        Isoelectric Point: 4.4507

>NTDB_id=392031 GA645_RS05500 WP_152220667.1 1183332..1184834(-) (rpoN/rpoN1) [Pseudomonas sp. SCB32]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEEGEDYDNSDPMADGAESGANGATSNSQDS
FQESTTPTADSLDEDQWAECIPSELPVDTAWEDIYQTSASSLPASDDDEWDFTARTSAGESLQSHLLWQLNLAPMSDTDR
LIALSIIDGINDDGYLEESLDDILASIDPELGVEMDEVQVVLRRVQQMEPAGVGARNLRECLLLQLRQMPANTYLLADAQ
KLVADHLDLLGSRDYSQLMRRMKLKEDELRETIELIQSLHPRPGSQIESGEAEYVVPDVIVRKHNERWLVELNQEAVPRL
RVNSQYAGLVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGEEAMKPLVLHDIAEAVGMH
ESTISRVTTQKFMHTPRGIFELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAESPKKPLSDSKIAGLLEAQGIQVARRT
VAKYRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=392031 GA645_RS05500 WP_152220667.1 1183332..1184834(-) (rpoN/rpoN1) [Pseudomonas sp. SCB32]
ATGAAACCATCGCTCGTCCTCAAGATGGGCCAGCAGCTGACGATGACCCCGCAGCTGCAACAGGCCATCCGACTGCTCCA
GCTCTCCACACTGGACCTTCAACAGGAAATCCAGGAAGCCCTGGAGTCCAACCCGATGCTGGAGCGCCAGGAAGAGGGCG
AGGATTACGACAACAGCGATCCCATGGCGGACGGCGCCGAGTCGGGCGCCAACGGCGCCACCAGCAACTCCCAGGACAGT
TTCCAGGAAAGCACCACTCCCACTGCCGACAGTCTCGATGAAGACCAGTGGGCCGAGTGCATTCCCAGCGAACTTCCAGT
TGATACCGCCTGGGAGGACATCTACCAGACCAGCGCCAGCAGCCTGCCCGCCAGCGACGACGACGAGTGGGACTTCACCG
CCCGTACCTCCGCGGGCGAAAGCCTGCAGAGCCACCTGCTCTGGCAGCTCAACCTGGCACCGATGTCCGACACTGACCGC
CTGATAGCCTTGAGCATCATCGACGGCATCAACGACGACGGGTACCTCGAAGAATCCCTCGACGACATCCTCGCCTCCAT
CGACCCCGAATTGGGTGTGGAGATGGACGAAGTGCAGGTTGTCCTGCGCCGCGTACAGCAGATGGAGCCCGCCGGCGTGG
GCGCGCGCAACCTGCGCGAATGCCTGTTGCTGCAGCTGCGCCAGATGCCGGCCAACACTTACCTGCTGGCCGACGCCCAG
AAACTGGTCGCCGACCACCTCGACCTGCTCGGCAGCCGCGACTACAGCCAGCTGATGCGCCGCATGAAGCTCAAGGAAGA
CGAGCTGCGCGAAACCATCGAGCTGATCCAGTCACTGCACCCGCGTCCGGGCTCGCAGATCGAGTCTGGTGAAGCCGAGT
ACGTGGTGCCGGATGTCATCGTTCGGAAACATAACGAGCGCTGGCTGGTGGAGCTCAACCAGGAAGCCGTGCCGCGCCTG
CGCGTCAACTCGCAGTATGCGGGCCTGGTGCGCCGCGCCGACTCCAGCGCCGACAACACCTTCATGCGCAACCAACTGCA
GGAAGCGCGCTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAGACACTGATGAAGGTGGCGACCCAGATCGTCGAACATC
AGCGTGGCTTCCTCGAATACGGCGAGGAGGCGATGAAGCCGTTGGTCCTGCATGACATCGCCGAAGCGGTAGGCATGCAC
GAGTCGACCATCTCGCGCGTCACCACGCAGAAATTCATGCACACGCCACGTGGCATCTTCGAGCTGAAGTACTTCTTCTC
CAGCCACGTCAGCACCTCCGAGGGTGGCGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTCGCGGCGG
AAAGTCCGAAAAAGCCATTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCACGTCGCACC
GTCGCCAAGTACCGGGAGTCCTTGGGGATTGCTCCCTCCAGCGAACGCAAGCGACTGGTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(132-326)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(340-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.348

100

0.482

Multiple sequence alignment