Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GBN93_RS15355 Genome accession   NZ_CP045103
Coordinates   3208301..3209101 (-) Length   266 a.a.
NCBI ID   WP_062031315.1    Uniprot ID   A0AAJ6LDX5
Organism   Acinetobacter johnsonii strain AJ413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3203301..3214101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN93_RS15335 (GBN93_15390) - 3204382..3205080 (-) 699 WP_005401704.1 YfgM family protein -
  GBN93_RS15340 (GBN93_15395) hisS 3205108..3206403 (-) 1296 WP_234964851.1 histidine--tRNA ligase -
  GBN93_RS15345 (GBN93_15400) ispG 3206400..3207515 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GBN93_RS15350 (GBN93_15405) - 3207534..3208310 (-) 777 WP_062031311.1 helix-turn-helix domain-containing protein -
  GBN93_RS15355 (GBN93_15410) pilF 3208301..3209101 (-) 801 WP_062031315.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GBN93_RS15360 (GBN93_15415) rlmN 3209118..3210353 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GBN93_RS15365 (GBN93_15420) ndk 3210476..3210907 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  GBN93_RS15370 (GBN93_15425) iscX 3211018..3211218 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  GBN93_RS15375 (GBN93_15430) - 3211309..3211860 (-) 552 WP_178904921.1 phosphatase PAP2 family protein -
  GBN93_RS15380 (GBN93_15435) - 3212053..3213333 (-) 1281 WP_234964852.1 glycosyltransferase family 4 protein -
  GBN93_RS15385 (GBN93_15440) - 3213474..3213890 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=391960 GBN93_RS15355 WP_062031315.1 3208301..3209101(-) (pilF) [Acinetobacter johnsonii strain AJ413]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYIQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=391960 GBN93_RS15355 WP_062031315.1 3208301..3209101(-) (pilF) [Acinetobacter johnsonii strain AJ413]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCGCAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATATACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

64.286

100

0.643

Multiple sequence alignment