Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GBN68_RS13870 Genome accession   NZ_CP045101
Coordinates   2855683..2856483 (-) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2850683..2861483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS13850 (GBN68_13870) - 2851764..2852462 (-) 699 WP_234304490.1 YfgM family protein -
  GBN68_RS13855 (GBN68_13875) hisS 2852490..2853785 (-) 1296 WP_004983518.1 histidine--tRNA ligase -
  GBN68_RS13860 (GBN68_13880) ispG 2853782..2854897 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GBN68_RS13865 (GBN68_13885) - 2854916..2855692 (-) 777 WP_130235008.1 helix-turn-helix domain-containing protein -
  GBN68_RS13870 (GBN68_13890) pilF 2855683..2856483 (-) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GBN68_RS13875 (GBN68_13895) rlmN 2856500..2857735 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GBN68_RS13880 (GBN68_13900) ndk 2857859..2858290 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  GBN68_RS13885 (GBN68_13905) iscX 2858401..2858601 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  GBN68_RS13890 (GBN68_13910) - 2858692..2859243 (-) 552 WP_148825482.1 phosphatase PAP2 family protein -
  GBN68_RS13895 (GBN68_13915) - 2859436..2860716 (-) 1281 WP_125274024.1 glycosyltransferase family 4 protein -
  GBN68_RS13900 (GBN68_13920) - 2860857..2861273 (-) 417 WP_234954289.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=391918 GBN68_RS13870 WP_004697674.1 2855683..2856483(-) (pilF) [Acinetobacter johnsonii strain AJ210]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=391918 GBN68_RS13870 WP_004697674.1 2855683..2856483(-) (pilF) [Acinetobacter johnsonii strain AJ210]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTGCTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647

Multiple sequence alignment