Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F9230_RS02420 Genome accession   NZ_CP045099
Coordinates   507278..508078 (+) Length   266 a.a.
NCBI ID   WP_234951134.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 502278..513078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9230_RS02390 (F9230_02400) - 502489..502905 (+) 417 WP_004983505.1 hypothetical protein -
  F9230_RS02395 (F9230_02405) - 503046..504326 (+) 1281 WP_234951133.1 glycosyltransferase family 4 protein -
  F9230_RS02400 (F9230_02410) - 504519..505070 (+) 552 WP_058870303.1 phosphatase PAP2 family protein -
  F9230_RS02405 (F9230_02415) iscX 505161..505361 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  F9230_RS02410 (F9230_02420) ndk 505472..505903 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  F9230_RS02415 (F9230_02425) rlmN 506026..507261 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  F9230_RS02420 (F9230_02430) pilF 507278..508078 (+) 801 WP_234951134.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  F9230_RS02425 (F9230_02435) - 508069..508845 (+) 777 WP_130235008.1 helix-turn-helix domain-containing protein -
  F9230_RS02430 (F9230_02440) ispG 508864..509985 (+) 1122 WP_086210453.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  F9230_RS02435 (F9230_02445) hisS 509989..511284 (+) 1296 WP_234951135.1 histidine--tRNA ligase -
  F9230_RS02440 (F9230_02450) - 511312..512010 (+) 699 WP_234951136.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29987.95 Da        Isoelectric Point: 8.7018

>NTDB_id=391858 F9230_RS02420 WP_234951134.1 507278..508078(+) (pilF) [Acinetobacter johnsonii strain AJ14]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVQAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=391858 F9230_RS02420 WP_234951134.1 507278..508078(+) (pilF) [Acinetobacter johnsonii strain AJ14]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAACATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCTCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAATACTATGAGCAGTTTGTGCAGGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGGGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_16)

(149-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

64.286

100

0.643

Multiple sequence alignment