Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GBN67_RS01740 Genome accession   NZ_CP045051
Coordinates   339939..340739 (+) Length   266 a.a.
NCBI ID   WP_004983514.1    Uniprot ID   A0A7H8SKN2
Organism   Acinetobacter johnsonii strain AJ312     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334939..345739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN67_RS01710 (GBN67_01705) - 335150..335566 (+) 417 WP_004983505.1 hypothetical protein -
  GBN67_RS01715 (GBN67_01710) - 335707..336987 (+) 1281 WP_234963435.1 glycosyltransferase family 4 protein -
  GBN67_RS01720 (GBN67_01715) - 337180..337731 (+) 552 WP_058870303.1 phosphatase PAP2 family protein -
  GBN67_RS01725 (GBN67_01720) iscX 337822..338022 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  GBN67_RS01730 (GBN67_01725) ndk 338133..338564 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  GBN67_RS01735 (GBN67_01730) rlmN 338687..339922 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GBN67_RS01740 (GBN67_01735) pilF 339939..340739 (+) 801 WP_004983514.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GBN67_RS01745 (GBN67_01740) - 340730..341506 (+) 777 WP_234963436.1 helix-turn-helix domain-containing protein -
  GBN67_RS01750 (GBN67_01745) ispG 341525..342640 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GBN67_RS01755 (GBN67_01750) hisS 342637..343932 (+) 1296 WP_234963437.1 histidine--tRNA ligase -
  GBN67_RS01760 (GBN67_01755) - 343960..344658 (+) 699 WP_005401704.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30016.01 Da        Isoelectric Point: 8.9759

>NTDB_id=391524 GBN67_RS01740 WP_004983514.1 339939..340739(+) (pilF) [Acinetobacter johnsonii strain AJ312]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=391524 GBN67_RS01740 WP_004983514.1 339939..340739(+) (pilF) [Acinetobacter johnsonii strain AJ312]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAACATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGGGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8SKN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647

Multiple sequence alignment