Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LM596_RS02920 Genome accession   NZ_CP045035
Coordinates   641876..643246 (+) Length   456 a.a.
NCBI ID   WP_003688908.1    Uniprot ID   J0KZM1
Organism   Liquorilactobacillus mali strain LM596     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 636876..648246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LM596_RS02895 (LM596_02895) - 637879..638553 (+) 675 WP_003688917.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  LM596_RS02900 (LM596_02900) - 638753..640087 (-) 1335 WP_003688916.1 aminopeptidase C -
  LM596_RS02905 (LM596_02905) rpiA 640162..640845 (-) 684 WP_003688913.1 ribose-5-phosphate isomerase RpiA -
  LM596_RS02910 (LM596_02910) - 640859..641152 (-) 294 WP_003688912.1 GNAT family N-acetyltransferase -
  LM596_RS02915 (LM596_02915) - 641256..641792 (+) 537 WP_003688910.1 hypothetical protein -
  LM596_RS02920 (LM596_02920) radA 641876..643246 (+) 1371 WP_003688908.1 DNA repair protein RadA Machinery gene
  LM596_RS02925 (LM596_02925) - 643268..644395 (+) 1128 WP_003688906.1 PIN/TRAM domain-containing protein -
  LM596_RS02930 (LM596_02930) gltX 644523..646007 (+) 1485 WP_003688904.1 glutamate--tRNA ligase -
  LM596_RS02935 (LM596_02935) cysS 646330..647751 (+) 1422 WP_003688902.1 cysteine--tRNA ligase -
  LM596_RS02940 (LM596_02940) - 647744..648148 (+) 405 WP_003688900.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49669.19 Da        Isoelectric Point: 8.0304

>NTDB_id=391401 LM596_RS02920 WP_003688908.1 641876..643246(+) (radA) [Liquorilactobacillus mali strain LM596]
MVKIKTQFVCQDCGYSSPRYLGRCPNCGAWNTLVEEVEKSNKTDKKTRVSFSGNSPKPQLIDEVNVQDNPRTTTASVELD
RVLGGGIVPGSLILIGGDPGIGKSTLLLQLSGQLARQELTVLYVSGEESAMQIKLRAQRLGVKGQQFYLYPETDMTSIHQ
YIDKLKPNFVVIDSVQTMQEPEMSSAVGSVAQIREVTAELMQIAKTNGITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHAYRILRAVKNRFGSTNEIGIFEMKEGGLYEVQNPSEIFLEERLSGATGSAIVVSLEGTRPILVEIQALVTPTAFG
NAKRTTTGLDHNRISLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSIVSSYRDAQSQATDCFIGELGLTG
EIRRVNRIEQRVAEAAKLGFKRVILPKNNLAGWTPPRNIKVVGVSTLSEALKVTFN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=391401 LM596_RS02920 WP_003688908.1 641876..643246(+) (radA) [Liquorilactobacillus mali strain LM596]
GTGGTCAAGATTAAAACACAGTTTGTGTGTCAAGATTGTGGGTATTCTTCACCCCGATATTTGGGTCGCTGTCCAAATTG
TGGTGCCTGGAATACTCTGGTTGAAGAGGTTGAGAAATCGAATAAAACTGATAAAAAAACGAGGGTTAGTTTTAGCGGTA
ACAGTCCTAAACCACAATTAATTGATGAAGTAAATGTGCAAGATAATCCGCGAACAACGACTGCATCAGTTGAACTTGAT
CGAGTTTTAGGCGGGGGGATCGTGCCGGGTTCGCTTATCCTAATAGGCGGTGATCCTGGAATTGGTAAGTCTACGCTATT
GCTTCAACTTTCGGGTCAGTTGGCAAGACAGGAATTAACAGTCTTGTATGTGTCTGGAGAAGAAAGCGCAATGCAGATCA
AACTGCGGGCACAGCGCTTGGGAGTAAAAGGGCAGCAATTTTATTTATATCCAGAGACTGATATGACAAGCATTCACCAA
TACATTGATAAGTTAAAACCCAATTTTGTAGTTATCGACTCAGTTCAGACCATGCAGGAACCTGAAATGTCTTCTGCAGT
TGGGAGTGTGGCACAGATCAGGGAGGTTACAGCCGAGTTAATGCAGATTGCAAAGACTAATGGCATAACAATTTTCATAG
TTGGTCATGTCACCAAGGGTGGAGCGATTGCTGGTCCCAAAATACTGGAACATATGGTTGATACGGTCCTATATTTTGAG
GGAGACTTGCACCACGCTTATCGAATTCTTAGAGCTGTAAAAAATAGATTTGGCTCGACAAATGAAATTGGAATTTTCGA
AATGAAAGAAGGAGGGCTGTACGAAGTTCAAAACCCTTCTGAGATTTTTTTGGAAGAACGACTCAGTGGAGCGACCGGCT
CTGCCATTGTTGTCTCTCTGGAAGGAACACGCCCAATTTTAGTTGAAATTCAGGCACTTGTTACACCAACTGCTTTTGGA
AATGCTAAGCGAACTACAACGGGATTAGATCACAACCGGATCTCTTTGATTATGGCCGTTCTTGAGAAACGTGCGGGATT
GCTTTTACAAAATCAAGATGCGTATTTAAAGGCCGCCGGCGGGGTGAAGCTAGATGAACCAGCCATCGACTTGGCGATTG
CAGTGAGCATTGTTTCAAGTTATCGCGATGCTCAATCGCAAGCAACTGACTGTTTTATTGGAGAACTCGGATTGACCGGT
GAAATCAGAAGAGTTAATCGGATTGAGCAAAGAGTTGCTGAGGCGGCTAAACTGGGGTTTAAAAGAGTAATTTTGCCTAA
AAATAATTTGGCTGGATGGACTCCCCCAAGAAACATAAAAGTTGTCGGTGTTTCGACTCTAAGTGAGGCACTGAAAGTAA
CTTTTAACTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

AAA domain (AAA_25)

(81-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J0KZM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.571

99.781

0.684
  radA Streptococcus mitis SK321

68.571

99.781

0.684
  radA Streptococcus pneumoniae Rx1

68.352

99.781

0.682
  radA Streptococcus pneumoniae D39

68.352

99.781

0.682
  radA Streptococcus pneumoniae R6

68.352

99.781

0.682
  radA Streptococcus pneumoniae TIGR4

68.352

99.781

0.682
  radA Bacillus subtilis subsp. subtilis str. 168

63.816

100

0.638

Multiple sequence alignment