Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A0J18_RS04625 Genome accession   NZ_CP045034
Coordinates   935663..937036 (+) Length   457 a.a.
NCBI ID   WP_004562734.1    Uniprot ID   A0A806TIP1
Organism   Limosilactobacillus fermentum strain USM 8633     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 930663..942036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0J18_RS04595 - 931288..931902 (-) 615 WP_024500694.1 sugar O-acetyltransferase -
  A0J18_RS04600 rpiA 931930..932616 (-) 687 WP_051132373.1 ribose-5-phosphate isomerase RpiA -
  A0J18_RS04605 tnpA 932649..933101 (-) 453 Protein_881 IS200/IS605 family transposase -
  A0J18_RS04610 - 933180..934430 (+) 1251 WP_021349622.1 RNA-guided endonuclease TnpB family protein -
  A0J18_RS04615 - 934585..934911 (-) 327 WP_035437471.1 GNAT family N-acetyltransferase -
  A0J18_RS04620 - 935111..935650 (+) 540 WP_004563338.1 dUTP diphosphatase -
  A0J18_RS04625 radA 935663..937036 (+) 1374 WP_004562734.1 DNA repair protein RadA Machinery gene
  A0J18_RS04630 - 937069..938202 (+) 1134 WP_004562735.1 PIN/TRAM domain-containing protein -
  A0J18_RS04635 gltX 938273..939769 (+) 1497 WP_012390831.1 glutamate--tRNA ligase -
  A0J18_RS04640 - 939837..940406 (+) 570 WP_004562737.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49381.79 Da        Isoelectric Point: 8.7358

>NTDB_id=391381 A0J18_RS04625 WP_004562734.1 935663..937036(+) (radA) [Limosilactobacillus fermentum strain USM 8633]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=391381 A0J18_RS04625 WP_004562734.1 935663..937036(+) (radA) [Limosilactobacillus fermentum strain USM 8633]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGAAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACAGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A806TIP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

68.212

99.125

0.676
  radA Streptococcus mitis NCTC 12261

67.991

99.125

0.674
  radA Streptococcus pneumoniae Rx1

67.991

99.125

0.674
  radA Streptococcus pneumoniae D39

67.991

99.125

0.674
  radA Streptococcus pneumoniae R6

67.991

99.125

0.674
  radA Streptococcus pneumoniae TIGR4

67.991

99.125

0.674
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637

Multiple sequence alignment