Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LKK75_RS02100 Genome accession   NZ_CP045033
Coordinates   404173..405549 (+) Length   458 a.a.
NCBI ID   WP_013853543.1    Uniprot ID   A0AAX3UA80
Organism   Lactobacillus kefiranofaciens subsp. kefiranofaciens strain LKK75     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 399173..410549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LKK75_RS02075 (LKK75_02075) - 400183..400567 (-) 385 Protein_402 hypothetical protein -
  LKK75_RS02080 (LKK75_02080) - 400774..401616 (-) 843 WP_013853539.1 RhaT/GlcU family sugar-proton symporter -
  LKK75_RS02085 (LKK75_02085) pepC 401760..403109 (-) 1350 WP_013853540.1 aminopeptidase C -
  LKK75_RS02090 (LKK75_02090) - 403173..403487 (-) 315 WP_013853541.1 hypothetical protein -
  LKK75_RS02095 (LKK75_02095) - 403622..404173 (+) 552 WP_013853542.1 dUTP diphosphatase -
  LKK75_RS02100 (LKK75_02100) radA 404173..405549 (+) 1377 WP_013853543.1 DNA repair protein RadA Machinery gene
  LKK75_RS02105 (LKK75_02105) gltX 405626..407125 (+) 1500 WP_013853544.1 glutamate--tRNA ligase -
  LKK75_RS02110 (LKK75_02110) cysS 407221..408654 (+) 1434 WP_013853545.1 cysteine--tRNA ligase -
  LKK75_RS02115 (LKK75_02115) - 408647..409090 (+) 444 WP_013853546.1 Mini-ribonuclease 3 -
  LKK75_RS02120 (LKK75_02120) rlmB 409077..409829 (+) 753 WP_013853547.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LKK75_RS02125 (LKK75_02125) - 409970..410518 (+) 549 WP_013853548.1 DNA-directed RNA polymerase subunit sigma -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50131.73 Da        Isoelectric Point: 6.9615

>NTDB_id=391362 LKK75_RS02100 WP_013853543.1 404173..405549(+) (radA) [Lactobacillus kefiranofaciens subsp. kefiranofaciens strain LKK75]
MARVKTQYKCHSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIKKTGINEPVKLDKVKAEKEDRIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAITMAVASSYTDKEISPIDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRVFIPRHNMHASLNSLGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=391362 LKK75_RS02100 WP_013853543.1 404173..405549(+) (radA) [Lactobacillus kefiranofaciens subsp. kefiranofaciens strain LKK75]
ATGGCACGAGTAAAAACACAATATAAATGTCATTCTTGTGGCTATATTTCCGCCAGCTATTTGGGACGTTGTCCTAATTG
TGGCGCGTGGAATCAGTTTGAAAAAGAAACTGAAGAAGTACAAAAGCGTTCTACAAAAGCGACTGCTAGCCGTTTAATCA
AAAAGACGGGTATCAATGAACCAGTTAAATTAGACAAGGTTAAGGCAGAAAAAGAAGACCGCATCCCTACTAAGTCAGAG
GAGTTAAATCGCGTCTTGGGTGGTGGCATCGTCCCAGGTTCGCTTGTTTTAATTGGCGGGGATCCTGGTATTGGCAAATC
GACCTTGATGTTGCAGATCATGAGCGACTTATCGGAAAAATATAAGGTGCTCTACGTTTCTGGGGAAGAATCAGCTAATC
AGATTAAATTAAGAGCCGATCGTTTAGGCGTGGGTCAAAGTAATATGTTGCTATATCCTGAAACAGACATGCATGATATT
CGCGAACAAATCAACGATGTAAAGCCTGATTTCGTAGTCATCGATTCGATTCAAACCATGAATGAGCCTAGTCTTGATTC
AATGACTGGTTCAGCTTCGCAAGTGCGTGAAGTGACCAGCGAACTCATGAAGATCGCTAAGATGGACGCCATTACCGTGT
TTGTCATTGGTCACGTGACTAAGGAAGGCGCGATTGCAGGACCGAAGATTTTGGAGCACATGGTGGATACTGTCCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGTATTTTGCACTCTGTGAAAAATCGTTTTGGCGCCGCAAACGAAATCGGCAT
GTTTGAGATGGAAAATGAAGGTTTAAAAGAAGTAACTAATCCATCATCGATTTTTCTTGATCAAAGACTGCCAAACTCTA
CTGGTTCAGCTGTGGTAGTTTCTCTAGAAGGAACTAGGCCGCTGCTTGCAGAAATTCAAGCATTAGTAACGCCAACTGCT
TTTGGCTATGCTAAAAGAACGACTTCAGGCATTAGTTTCAATAAGGCTTCATTATTATTGGCCGTGCTGGAAAAACGAGG
CAATTTAATGTTGCAAAACCAGGATGTTTATTTAACTGCAACTGGCGGCATTAAATTAAATGAACCAGCGATTGATTTGG
CTATTACAATGGCAGTAGCTTCAAGCTACACTGATAAAGAAATTTCGCCTATAGATTGTTTTGTAGGTGAAGTAGGGCTA
ACCGGCGAAGTTCGCCGCGTTGATAAAATTGAAGCACGAGTAAAAGAAGCCGCTAAAGTGGGCTTCAAGCGTGTTTTTAT
TCCGCGGCATAATATGCATGCTAGTTTAAACAGCTTAGGCATTGAGGTTATTCCGGTTTCCAGCATTCCACAGGCGTTAA
AATTAGTTTTTGGTTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.338

100

0.555

Multiple sequence alignment