Detailed information NTG0391344

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: F9800_RS32810
• Genome accession: NZ_CP045030
• Coordinates: 5144342..5145691 (-)
• Length: 449 a.a.
• NCBI ID: WP_002083580.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain JW-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5139342..5150691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F9800_RS32790 (F9800_32820)	secA	5139670..5142177 (-)	2508	WP_000579374.1	preprotein translocase subunit SecA	-
F9800_RS32795 (F9800_32825)	hpf	5142450..5142992 (-)	543	WP_000671187.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
F9800_RS32800 (F9800_32830)	cspC	5143314..5143511 (-)	198	WP_001990088.1	cold shock protein CspC	-
F9800_RS32805 (F9800_32835)	-	5143638..5144342 (-)	705	WP_003255239.1	ComF family protein	-
F9800_RS32810 (F9800_32840)	comFA	5144342..5145691 (-)	1350	WP_002083580.1	ATP-dependent helicase ComFA	Machinery gene
F9800_RS32815 (F9800_32845)	-	5145819..5147204 (-)	1386	WP_000499483.1	NlpC/P60 family protein	-
F9800_RS32820 (F9800_32850)	-	5147353..5147667 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
F9800_RS32825 (F9800_32855)	-	5147840..5148682 (-)	843	WP_000684726.1	DegV family protein	-
F9800_RS32830 (F9800_32860)	-	5148919..5149554 (+)	636	WP_000926669.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51166.05 Da
• Isoelectric Point: 10.0230

>NTDB_id=391344 F9800_RS32810 WP_002083580.1 5144342..5145691(-) (comFA) [Bacillus thuringiensis strain JW-1]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=391344 F9800_RS32810 WP_002083580.1 5144342..5145691(-) (comFA) [Bacillus thuringiensis strain JW-1]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.131	98.441	0.503
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37