Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   F9800_RS26650 Genome accession   NZ_CP045030
Coordinates   4030930..4031364 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain JW-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4025930..4036364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9800_RS26635 (F9800_26670) - 4027108..4027665 (+) 558 WP_000062064.1 PadR family transcriptional regulator -
  F9800_RS26640 (F9800_26675) metE 4028178..4030466 (+) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  F9800_RS26645 (F9800_26680) - 4030504..4030893 (-) 390 WP_000424022.1 competence protein ComJ -
  F9800_RS26650 (F9800_26685) nucA/comI 4030930..4031364 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  F9800_RS26655 (F9800_26690) - 4031593..4032342 (+) 750 WP_000388468.1 DUF3967 domain-containing protein -
  F9800_RS26660 (F9800_26695) kinB 4032381..4033655 (-) 1275 WP_000420367.1 sporulation sensor histidine kinase KinB -
  F9800_RS26665 (F9800_26700) - 4033966..4034166 (+) 201 WP_000929277.1 hypothetical protein -
  F9800_RS26670 (F9800_26705) - 4034288..4035451 (+) 1164 WP_000434585.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=391330 F9800_RS26650 WP_000811494.1 4030930..4031364(-) (nucA/comI) [Bacillus thuringiensis strain JW-1]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=391330 F9800_RS26650 WP_000811494.1 4030930..4031364(-) (nucA/comI) [Bacillus thuringiensis strain JW-1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment