Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OCEPR_RS07015 Genome accession   NC_014761
Coordinates   1421267..1421908 (+) Length   213 a.a.
NCBI ID   WP_013458015.1    Uniprot ID   E4U915
Organism   Oceanithermus profundus DSM 14977     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1416267..1426908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCEPR_RS13160 - 1417061..1417237 (-) 177 WP_245544451.1 hypothetical protein -
  OCEPR_RS13200 (Ocepr_1385) - 1417213..1418496 (+) 1284 WP_342626188.1 HD-GYP domain-containing protein -
  OCEPR_RS07000 (Ocepr_1386) - 1418382..1418966 (+) 585 WP_148229278.1 DUF5317 domain-containing protein -
  OCEPR_RS07005 (Ocepr_1387) - 1418998..1420023 (+) 1026 WP_013458013.1 isocitrate/isopropylmalate dehydrogenase family protein -
  OCEPR_RS07010 (Ocepr_1388) pilM 1420129..1421274 (+) 1146 WP_013458014.1 type IV pilus assembly protein PilM Machinery gene
  OCEPR_RS07015 (Ocepr_1389) pilN 1421267..1421908 (+) 642 WP_013458015.1 flagellar protein FliT Machinery gene
  OCEPR_RS07020 (Ocepr_1390) pilO 1421898..1422488 (+) 591 WP_013458016.1 type 4a pilus biogenesis protein PilO -
  OCEPR_RS07025 (Ocepr_1391) - 1422485..1423342 (+) 858 WP_013458017.1 hypothetical protein -
  OCEPR_RS07030 (Ocepr_1392) - 1423345..1424895 (+) 1551 WP_041554095.1 secretin N-terminal domain-containing protein -
  OCEPR_RS07035 (Ocepr_1393) aroC 1424961..1426118 (+) 1158 WP_013458019.1 chorismate synthase -
  OCEPR_RS07040 (Ocepr_1394) - 1426118..1426678 (+) 561 WP_013458020.1 shikimate kinase -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 24487.03 Da        Isoelectric Point: 10.5548

>NTDB_id=39123 OCEPR_RS07015 WP_013458015.1 1421267..1421908(+) (pilN) [Oceanithermus profundus DSM 14977]
MIRLNLLPKRLRKRVEPGWWRLTAIIFPLVVFAVVAMIHMSVSNQVGQLEDERDKLKIEVQALQPYIQRQQELTRRKAEL
EKIINVDREIKARFIPWSDNLARFINQIPRRGGRFEVFLRSVNTRLVPQETRANLAEQGLYDRKPVAVEFNLQGEARNEN
ALARFVQAFEDNPNFGINFQQGSLNENGGFDFTATVAITESGAKPEGGERSAR

Nucleotide


Download         Length: 642 bp        

>NTDB_id=39123 OCEPR_RS07015 WP_013458015.1 1421267..1421908(+) (pilN) [Oceanithermus profundus DSM 14977]
TTGATTAGACTCAACCTCCTGCCCAAGCGCTTGCGCAAGCGGGTGGAGCCCGGCTGGTGGCGCCTCACGGCCATCATCTT
CCCCCTCGTCGTCTTCGCCGTCGTGGCCATGATCCACATGAGCGTGAGCAACCAGGTCGGCCAGCTCGAGGACGAGCGCG
ACAAACTCAAGATCGAGGTCCAGGCCCTGCAGCCCTACATTCAACGGCAGCAGGAGCTGACCCGGCGCAAGGCGGAGCTG
GAAAAGATCATCAACGTCGACCGAGAGATCAAGGCACGCTTCATCCCCTGGTCCGACAACCTCGCGCGCTTCATCAACCA
GATCCCCCGCCGGGGCGGCCGCTTCGAGGTCTTCCTGCGCTCGGTCAACACCCGGCTCGTGCCCCAGGAGACCCGGGCCA
ACCTCGCGGAACAGGGCCTCTACGACCGCAAGCCCGTCGCCGTCGAGTTCAACCTGCAGGGCGAGGCCCGCAACGAGAAC
GCGCTGGCCCGATTCGTCCAGGCCTTCGAGGACAACCCCAACTTCGGCATCAACTTCCAGCAGGGCTCGCTCAACGAAAA
CGGCGGCTTCGACTTCACCGCCACCGTAGCCATCACCGAGTCCGGCGCCAAGCCCGAGGGGGGTGAGCGCAGTGCTCGCT
AA

Domains

Predicted by InterproScan.

PilN biogenesis protein dimerization domain (PilN_bio_d)

(97-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4U915

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Thermus thermophilus HB27

49.758

97.183

0.484

Multiple sequence alignment