Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   DDE73_RS25610 Genome accession   NZ_CP044978
Coordinates   4952470..4953216 (-) Length   248 a.a.
NCBI ID   WP_000487009.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain BT62     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4947470..4958216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDE73_RS25595 (DDE73_25605) dnaG 4948231..4950027 (-) 1797 WP_103622321.1 DNA primase -
  DDE73_RS31430 - 4950109..4950252 (-) 144 WP_000479733.1 hypothetical protein -
  DDE73_RS25600 (DDE73_25610) - 4950888..4951700 (-) 813 WP_000368944.1 pyruvate, water dikinase regulatory protein -
  DDE73_RS25605 (DDE73_25615) - 4951730..4952362 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  DDE73_RS25610 (DDE73_25620) recO 4952470..4953216 (-) 747 WP_000487009.1 DNA repair protein RecO Machinery gene
  DDE73_RS25615 (DDE73_25625) - 4953250..4953393 (-) 144 WP_000883924.1 YqzL family protein -
  DDE73_RS25620 (DDE73_25630) era 4953570..4954475 (-) 906 WP_001080230.1 GTPase Era -
  DDE73_RS25625 (DDE73_25635) - 4954468..4954866 (-) 399 WP_001086283.1 cytidine deaminase -
  DDE73_RS25630 (DDE73_25640) - 4954966..4955319 (-) 354 WP_000715464.1 diacylglycerol kinase family protein -
  DDE73_RS25635 (DDE73_25645) ybeY 4955316..4955786 (-) 471 WP_001050397.1 rRNA maturation RNase YbeY -
  DDE73_RS25640 (DDE73_25650) - 4955783..4957927 (-) 2145 WP_000091510.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28618.21 Da        Isoelectric Point: 8.0566

>NTDB_id=391123 DDE73_RS25610 WP_000487009.1 4952470..4953216(-) (recO) [Bacillus thuringiensis strain BT62]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=391123 DDE73_RS25610 WP_000487009.1 4952470..4953216(-) (recO) [Bacillus thuringiensis strain BT62]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAAATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCATTTTCTAATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTAAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCATATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGCCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment