Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ51_RS06640 Genome accession   NZ_CP053247
Coordinates   1323837..1324277 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SCU-482     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1318837..1329277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ51_RS06620 (HHJ51_06620) aroP 1319835..1321208 (+) 1374 WP_023145664.1 aromatic amino acid transporter AroP -
  HHJ51_RS06625 (HHJ51_06625) ampE 1321251..1322105 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HHJ51_RS06630 (HHJ51_06630) ampD 1322102..1322653 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ51_RS06635 (HHJ51_06635) nadC 1322741..1323634 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ51_RS06640 (HHJ51_06640) pilA 1323837..1324277 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ51_RS06645 (HHJ51_06645) pilB 1324287..1325672 (+) 1386 WP_001025168.1 type II secretion system protein GspE Machinery gene
  HHJ51_RS06650 (HHJ51_06650) hofC 1325662..1326864 (+) 1203 WP_000157287.1 protein transport protein HofC -
  HHJ51_RS06655 (HHJ51_06655) guaC 1326899..1327942 (-) 1044 WP_001217337.1 GMP reductase -
  HHJ51_RS25825 - 1328098..1328142 (-) 45 WP_120795372.1 protein YacM -
  HHJ51_RS06660 (HHJ51_06660) coaE 1328167..1328787 (+) 621 WP_001269513.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=390977 HHJ51_RS06640 WP_000360904.1 1323837..1324277(+) (pilA) [Escherichia coli strain SCU-482]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=390977 HHJ51_RS06640 WP_000360904.1 1323837..1324277(+) (pilA) [Escherichia coli strain SCU-482]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363