Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ54_RS12690 Genome accession   NZ_CP053245
Coordinates   2724561..2725001 (-) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli strain SCU-485     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719561..2730001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ54_RS12670 (HHJ54_12670) coaE 2720051..2720671 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  HHJ54_RS23120 - 2720696..2720740 (+) 45 WP_120795372.1 protein YacM -
  HHJ54_RS12675 (HHJ54_12675) guaC 2720896..2721939 (+) 1044 WP_001217338.1 GMP reductase -
  HHJ54_RS12680 (HHJ54_12680) hofC 2721974..2723176 (-) 1203 WP_000157292.1 protein transport protein HofC -
  HHJ54_RS12685 (HHJ54_12685) pilB 2723166..2724551 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  HHJ54_RS12690 (HHJ54_12690) pilA 2724561..2725001 (-) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ54_RS12695 (HHJ54_12695) nadC 2725205..2726098 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ54_RS12700 (HHJ54_12700) ampD 2726186..2726737 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ54_RS12705 (HHJ54_12705) ampE 2726734..2727588 (+) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  HHJ54_RS12710 (HHJ54_12710) aroP 2727631..2729001 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=390942 HHJ54_RS12690 WP_000360915.1 2724561..2725001(-) (pilA) [Escherichia coli strain SCU-485]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=390942 HHJ54_RS12690 WP_000360915.1 2724561..2725001(-) (pilA) [Escherichia coli strain SCU-485]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363