Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LF145_RS01605 Genome accession   NZ_CP044534
Coordinates   361615..362988 (+) Length   457 a.a.
NCBI ID   WP_057748334.1    Uniprot ID   A0A0R1PIT9
Organism   Limosilactobacillus frumenti strain LF145     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 356615..367988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF145_RS01580 (LF145_01580) - 357129..358748 (-) 1620 WP_057748323.1 malolactic enzyme -
  LF145_RS01585 (LF145_01585) - 358873..359760 (+) 888 WP_057748326.1 LysR family transcriptional regulator -
  LF145_RS01590 (LF145_01590) rpiA 359796..360488 (-) 693 WP_057748329.1 ribose-5-phosphate isomerase RpiA -
  LF145_RS01595 (LF145_01595) - 360506..360799 (-) 294 WP_057748405.1 GNAT family N-acetyltransferase -
  LF145_RS01600 (LF145_01600) - 360897..361433 (+) 537 WP_057748332.1 dUTP diphosphatase -
  LF145_RS01605 (LF145_01605) radA 361615..362988 (+) 1374 WP_057748334.1 DNA repair protein RadA Machinery gene
  LF145_RS01610 (LF145_01610) - 362996..364150 (+) 1155 WP_057748337.1 PIN/TRAM domain-containing protein -
  LF145_RS01615 (LF145_01615) - 364268..365929 (+) 1662 WP_057748339.1 formate--tetrahydrofolate ligase -
  LF145_RS01620 (LF145_01620) - 366129..366476 (-) 348 WP_057748342.1 glycine cleavage system protein H -
  LF145_RS01625 (LF145_01625) - 366490..367518 (-) 1029 WP_057748345.1 lipoate--protein ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49970.29 Da        Isoelectric Point: 8.7033

>NTDB_id=390866 LF145_RS01605 WP_057748334.1 361615..362988(+) (radA) [Limosilactobacillus frumenti strain LF145]
MARVKTQYICQNCGYNSPRYLGRCPNCGEWNTFVEEQVETGAKATKQATTTLTGLVAKPQKISDINSHEMPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESATQIKMRAERLNVAADDFYIYPETNMDSIR
NTIRELKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGALAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTRPSDAFVGEVGLT
GEIRRVNRIEQRVAEAQKLGFERIFVPKNNLKGWQAPSGIEVIGVATLRQALRLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=390866 LF145_RS01605 WP_057748334.1 361615..362988(+) (radA) [Limosilactobacillus frumenti strain LF145]
ATGGCCAGAGTAAAGACACAGTATATTTGTCAAAATTGCGGGTACAATTCACCACGATATTTAGGACGCTGCCCAAACTG
TGGTGAGTGGAATACCTTTGTTGAAGAACAAGTTGAAACTGGTGCTAAGGCAACGAAACAAGCGACAACGACCTTGACTG
GTTTAGTTGCTAAGCCGCAAAAAATTAGTGACATTAATAGTCATGAAATGCCTAGAGTCAAGACTAAATTAAACGAATTA
AACCGAGTCCTAGGGGGCGGAATTGTTCCAGGGTCTTTGATTTTAATCGGTGGTGATCCGGGAATTGGGAAATCGACCCT
ATTGCTTCAAGTATCCGGACAATTAAGTGATGAACATCACCGGGTCTTGTATGTTTCTGGGGAAGAAAGTGCCACTCAAA
TTAAGATGCGGGCGGAACGGCTGAATGTGGCCGCAGATGACTTTTATATTTATCCTGAAACAAATATGGATAGCATTCGA
AACACCATTCGGGAGTTAAAGCCTGAATACGTCGTGATTGACTCTGTACAAACAATGCAAGCCACGGATGTTTCATCGGC
GATTGGCTCTGTTTCCCAGATCCGGGAAGTGACTGCCCAGTTAATGCAGATTGCCAAGAGCAACAACATCACTATCTTTG
TGGTCGGGCACGTAACAAAAGGTGGTGCCCTAGCAGGGCCAAAGATTTTGGAGCACATGGTAGATACCGTGCTGTACTTT
GAAGGTGACCTTCACCATACTTATCGGATTCTACGATCAGTCAAAAACCGGTTTGGGTCCACTAATGAACTGGGTATTTT
TGAAATGCATACTAATGGGTTGACCGAAGTTAAAAACCCATCCGAAATCTTTTTGGAAGAACGACTCAAGGATGCTACCG
GTTCAGCAGTGGTAGTATCGCTCGAAGGAACCCGGCCAATCCTCGTTGAAATTCAGGCCCTGGTTACGCCAACTGTTTAC
GGTAATGCCCAGCGAACTGCTACCGGTTTAAGCCGAAACCGTGTATCATTAATAATGGCCGTTTTGGAGAAACGTGCTAA
CTTAATGCTGCAAAACCAAGATGCTTATTTAAAGGCGGCTGGTGGTGTTAAACTGGACGAACCTGCCATTGATTTAGCAA
TTGCTGTCAGCATCGCTTCTAGTTATCGTGACAAAGGGACCCGTCCATCGGATGCTTTTGTTGGCGAAGTTGGTTTAACA
GGAGAAATCCGCCGAGTGAACCGCATCGAACAGCGGGTCGCCGAAGCACAGAAACTCGGTTTTGAGCGGATCTTTGTTCC
CAAAAATAACTTAAAGGGATGGCAAGCACCATCCGGGATTGAAGTAATTGGGGTGGCAACGTTACGTCAAGCATTGCGGT
TGGCATTAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0R1PIT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

66.667

99.125

0.661
  radA Streptococcus pneumoniae D39

66.667

99.125

0.661
  radA Streptococcus pneumoniae R6

66.667

99.125

0.661
  radA Streptococcus pneumoniae TIGR4

66.667

99.125

0.661
  radA Streptococcus mitis NCTC 12261

66.667

99.125

0.661
  radA Streptococcus mitis SK321

66.667

99.125

0.661
  radA Bacillus subtilis subsp. subtilis str. 168

63.239

100

0.632

Multiple sequence alignment