Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F7R97_RS02485 Genome accession   NZ_CP044517
Coordinates   535289..536089 (+) Length   266 a.a.
NCBI ID   WP_218650178.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 31FS3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530289..541089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7R97_RS02460 (F7R97_02480) - 531199..532497 (+) 1299 WP_001991191.1 glycosyltransferase family 4 protein -
  F7R97_RS02465 (F7R97_02485) - 532522..533073 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  F7R97_RS02470 (F7R97_02490) iscX 533173..533373 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  F7R97_RS02475 (F7R97_02495) ndk 533487..533918 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  F7R97_RS02480 (F7R97_02500) rlmN 534041..535273 (+) 1233 WP_031959661.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  F7R97_RS02485 (F7R97_02505) pilF 535289..536089 (+) 801 WP_218650178.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  F7R97_RS02490 (F7R97_02510) - 536080..536871 (+) 792 WP_179007037.1 helix-turn-helix domain-containing protein -
  F7R97_RS02495 (F7R97_02515) ispG 536893..538008 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  F7R97_RS02500 (F7R97_02520) hisS 538005..539297 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  F7R97_RS02505 (F7R97_02525) - 539326..540024 (+) 699 WP_179007040.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30108.51 Da        Isoelectric Point: 9.3928

>NTDB_id=390733 F7R97_RS02485 WP_218650178.1 535289..536089(+) (pilF) [Acinetobacter baumannii strain 31FS3-2]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYVQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=390733 F7R97_RS02485 WP_218650178.1 535289..536089(+) (pilF) [Acinetobacter baumannii strain 31FS3-2]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACACAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGTTCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment