Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   F8M43_RS13195 Genome accession   NZ_CP044498
Coordinates   2534392..2534802 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain ms-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2529392..2539802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS13165 - 2529514..2529654 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  F8M43_RS13170 yqeF 2529960..2530691 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  F8M43_RS13175 cwlH 2530943..2531695 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  F8M43_RS13180 yqeD 2531882..2532508 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  F8M43_RS13185 gnd 2532527..2533395 (-) 869 Protein_2540 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  F8M43_RS13190 yqeB 2533637..2534359 (+) 723 WP_010886572.1 hypothetical protein -
  F8M43_RS13195 nucA/comI 2534392..2534802 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  F8M43_RS13200 - 2534998..2535345 (+) 348 Protein_2543 sigma-70 family RNA polymerase sigma factor -
  F8M43_RS13205 spoIVCA 2535376..2536836 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  F8M43_RS21735 - 2536794..2536972 (-) 179 Protein_2545 hypothetical protein -
  F8M43_RS13215 arsC 2537327..2537746 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  F8M43_RS13220 acr3 2537758..2538798 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  F8M43_RS13225 arsK 2538821..2539261 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  F8M43_RS13230 arsR 2539322..2539639 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=390575 F8M43_RS13195 WP_009967785.1 2534392..2534802(-) (nucA/comI) [Bacillus subtilis strain ms-2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=390575 F8M43_RS13195 WP_009967785.1 2534392..2534802(-) (nucA/comI) [Bacillus subtilis strain ms-2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment