Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   F8M43_RS12945 Genome accession   NZ_CP044498
Coordinates   2490986..2491753 (-) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis strain ms-2     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2485986..2496753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS12925 ppsR 2486160..2486972 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  F8M43_RS12930 ccpN 2486998..2487636 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  F8M43_RS12935 glyS 2487769..2489808 (-) 2040 WP_029726706.1 glycine--tRNA ligase subunit beta -
  F8M43_RS12940 glyQ 2489801..2490688 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  F8M43_RS12945 recO 2490986..2491753 (-) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  F8M43_RS12950 yqzL 2491790..2491933 (-) 144 WP_003230053.1 YqzL family protein -
  F8M43_RS12955 era 2492081..2492986 (-) 906 WP_003230051.1 GTPase Era -
  F8M43_RS12960 - 2492967..2493377 (-) 411 WP_003230049.1 cytidine deaminase -
  F8M43_RS12965 unpK 2493496..2493867 (-) 372 WP_004398824.1 diacylglycerol kinase family protein -
  F8M43_RS12970 ybeY 2493848..2494321 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  F8M43_RS12975 pgpH 2494322..2496457 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=390571 F8M43_RS12945 WP_003230055.1 2490986..2491753(-) (recO) [Bacillus subtilis strain ms-2]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=390571 F8M43_RS12945 WP_003230055.1 2490986..2491753(-) (recO) [Bacillus subtilis strain ms-2]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment