Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   F8M43_RS01990 Genome accession   NZ_CP044498
Coordinates   370398..370847 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis strain ms-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365398..375847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS01955 yckA 365549..366229 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  F8M43_RS01960 yckB 366239..367102 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  F8M43_RS01965 - 367264..367461 (+) 198 WP_119122841.1 hypothetical protein -
  F8M43_RS01970 yckC 367480..367935 (+) 456 WP_003246356.1 RDD family protein -
  F8M43_RS01975 yckD 368017..368349 (+) 333 WP_003246247.1 YckD family protein -
  F8M43_RS01980 bglC 368503..369936 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  F8M43_RS01985 nin/comJ 369973..370371 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  F8M43_RS01990 nucA/comI 370398..370847 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  F8M43_RS01995 tlpC 371015..372736 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  F8M43_RS02000 hxlB 372847..373404 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  F8M43_RS02005 hxlA 373410..374042 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  F8M43_RS02010 hxlR 374275..374637 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=390530 F8M43_RS01990 WP_003246436.1 370398..370847(-) (nucA/comI) [Bacillus subtilis strain ms-2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=390530 F8M43_RS01990 WP_003246436.1 370398..370847(-) (nucA/comI) [Bacillus subtilis strain ms-2]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment