Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   F8M43_RS00020 Genome accession   NZ_CP044498
Coordinates   3028..4140 (+) Length   370 a.a.
NCBI ID   WP_003226809.1    Uniprot ID   -
Organism   Bacillus subtilis strain ms-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS00005 dnaA 1..1341 (+) 1341 WP_003242674.1 chromosomal replication initiator protein DnaA -
  F8M43_RS00010 dnaN 1530..2666 (+) 1137 WP_003226811.1 DNA polymerase III subunit beta -
  F8M43_RS00015 rlbA 2797..3012 (+) 216 WP_003226810.1 ribosome maturation protein RlbA -
  F8M43_RS00020 recF 3028..4140 (+) 1113 WP_003226809.1 DNA replication/repair protein RecF Machinery gene
  F8M43_RS00025 remB 4158..4403 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  F8M43_RS00030 gyrB 4458..6374 (+) 1917 WP_003226808.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  F8M43_RS00035 gyrA 6585..9050 (+) 2466 WP_014475555.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42318.37 Da        Isoelectric Point: 7.3661

>NTDB_id=390523 F8M43_RS00020 WP_003226809.1 3028..4140(+) (recF) [Bacillus subtilis strain ms-2]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALEVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=390523 F8M43_RS00020 WP_003226809.1 3028..4140(+) (recF) [Bacillus subtilis strain ms-2]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCCGTCTACCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TTTCAAGAGGGCTTGAAGAACTGACCCTGAAATACCATACAGCTCTTGAGGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCACATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.73

100

0.997

Multiple sequence alignment