Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LA749_RS02140 Genome accession   NZ_CP044496
Coordinates   416038..417414 (+) Length   458 a.a.
NCBI ID   WP_056969481.1    Uniprot ID   A0A5P5ZGX5
Organism   Lactobacillus acetotolerans strain LA749     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 411038..422414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA749_RS02110 (LA749_02110) - 411661..411843 (+) 183 WP_054681182.1 hypothetical protein -
  LA749_RS02115 (LA749_02115) - 411855..412532 (+) 678 WP_056969489.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  LA749_RS02120 (LA749_02120) - 412638..413573 (+) 936 WP_056969487.1 AAA family ATPase -
  LA749_RS02125 (LA749_02125) pepC 413626..414975 (-) 1350 WP_056969485.1 aminopeptidase C -
  LA749_RS02130 (LA749_02130) - 415056..415358 (-) 303 WP_054681179.1 hypothetical protein -
  LA749_RS02135 (LA749_02135) - 415480..416037 (+) 558 WP_056969483.1 dUTP diphosphatase -
  LA749_RS02140 (LA749_02140) radA 416038..417414 (+) 1377 WP_056969481.1 DNA repair protein RadA Machinery gene
  LA749_RS02145 (LA749_02145) gltX 417492..418991 (+) 1500 WP_056969479.1 glutamate--tRNA ligase -
  LA749_RS02150 (LA749_02150) cysS 419091..420521 (+) 1431 WP_056969477.1 cysteine--tRNA ligase -
  LA749_RS02155 (LA749_02155) - 420508..420951 (+) 444 WP_056969474.1 Mini-ribonuclease 3 -
  LA749_RS02160 (LA749_02160) rlmB 420938..421693 (+) 756 WP_056969472.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LA749_RS02165 (LA749_02165) - 421820..422395 (+) 576 WP_056969470.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50324.10 Da        Isoelectric Point: 8.5379

>NTDB_id=390478 LA749_RS02140 WP_056969481.1 416038..417414(+) (radA) [Lactobacillus acetotolerans strain LA749]
MARIKTKYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSAKASPSRLIKKTGINEPVKLDKVKAEKDDRIATKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSKKYKVLYVSGEESASQIKLRANRLGLGASDMLLYPETDMHDI
REQINDVQPDFVVIDSIQTMNEPSLDTMTGSASQVREVTSELMKIAKMDAITIFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDKHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDERLPRSTGSSVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAITMSVASSYTNKEISATDCFVGEVGL
TGEVRRVNKIQARVNEAAKVGFKRIFIPRHNMYKGLKDLEIEVVPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=390478 LA749_RS02140 WP_056969481.1 416038..417414(+) (radA) [Lactobacillus acetotolerans strain LA749]
ATGGCCCGTATAAAAACCAAATATAAGTGCCGTTCTTGTGGTTATATTTCTGCAAGTTATTTAGGGCGCTGTCCTAATTG
TGGCGCCTGGAATCAATTTGAAAAGGAAACGGAAGAAGTTCAAAAGCGTTCTGCCAAGGCCAGCCCCAGTCGGTTGATTA
AGAAAACAGGTATAAATGAGCCTGTTAAGTTAGATAAAGTTAAAGCGGAAAAAGACGACCGGATAGCCACTAAATCTGAA
GAATTGAACCGTGTTTTAGGTGGTGGTATTGTACCCGGCTCACTTGTATTAATCGGTGGGGATCCTGGAATAGGTAAATC
TACTTTGATGTTACAAATCATGAGTGATCTATCTAAAAAATATAAAGTTCTTTATGTTTCTGGAGAGGAATCAGCTAGTC
AGATTAAGCTGCGTGCCAATCGTTTAGGCTTAGGCGCTAGTGATATGCTTTTATATCCAGAAACAGATATGCACGATATT
CGTGAGCAAATTAATGATGTACAGCCTGATTTCGTTGTAATTGATTCTATTCAGACCATGAATGAGCCAAGTTTAGATAC
AATGACTGGCTCAGCTTCACAGGTTCGGGAAGTAACCAGTGAGTTGATGAAAATTGCTAAAATGGACGCAATTACTATTT
TTGTTATTGGCCATGTTACTAAAGAGGGAGCAATTGCTGGGCCAAAAATCATGGAGCATATGGTGGATACCGTTCTTTAC
TTTGAAGGAGATAAACACCACTCCTATCGAATCTTACACTCTGTAAAAAATCGTTTTGGTGCTGCTAATGAAATTGGGAT
GTTTGAGATGGAAAATGAGGGCTTAAAAGAAGTTACTAATCCTTCGTCAATCTTTCTTGACGAACGTTTACCGCGTTCGA
CAGGTTCATCCGTTGTGGTTTCACTTGAAGGAACACGACCTCTGTTAGCTGAAATTCAGGCGCTAGTCACACCAACAGCG
TTTGGCTATGCTAAGAGGACTACATCAGGTATTGATTATAATCGAGCCGCCCTTCTTTTAGCGGTACTTGAAAAAAGGGG
CAACCTTATGTTGCAAAATCAGGATGTATATCTTACCGCGACCGGTGGTATTAAATTAAACGAACCAGCGATTGATCTAG
CTATAACGATGTCTGTGGCATCGAGTTATACTAACAAAGAAATAAGTGCTACAGATTGTTTTGTAGGCGAAGTTGGTTTA
ACCGGAGAAGTTCGGCGGGTAAATAAAATTCAGGCGCGAGTTAATGAGGCAGCTAAGGTTGGGTTTAAACGGATCTTTAT
TCCTCGTCACAATATGTATAAGGGATTAAAAGATTTGGAAATTGAAGTTGTTCCCGTTTCGAGTATCCCGCAAGCTTTAA
AGCTTGTTTTTGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P5ZGX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

58.425

99.782

0.583
  radA Streptococcus mitis NCTC 12261

58.425

99.782

0.583
  radA Streptococcus pneumoniae Rx1

58.425

99.782

0.583
  radA Streptococcus pneumoniae D39

58.425

99.782

0.583
  radA Streptococcus pneumoniae R6

58.425

99.782

0.583
  radA Streptococcus pneumoniae TIGR4

58.425

99.782

0.583
  radA Bacillus subtilis subsp. subtilis str. 168

54.684

100

0.548

Multiple sequence alignment