Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FSC11_RS12200 Genome accession   NZ_CP044483
Coordinates   2542050..2542850 (-) Length   266 a.a.
NCBI ID   WP_163167152.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain HZE30-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537050..2547850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC11_RS12180 (FSC11_12190) - 2538095..2538793 (-) 699 WP_004889998.1 YfgM family protein -
  FSC11_RS12185 (FSC11_12195) hisS 2538824..2540119 (-) 1296 WP_163167148.1 histidine--tRNA ligase -
  FSC11_RS12190 (FSC11_12200) ispG 2540123..2541244 (-) 1122 WP_004890004.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FSC11_RS12195 (FSC11_12205) - 2541268..2542059 (-) 792 WP_163167150.1 helix-turn-helix domain-containing protein -
  FSC11_RS12200 (FSC11_12210) pilF 2542050..2542850 (-) 801 WP_163167152.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FSC11_RS12205 (FSC11_12215) rlmN 2542865..2544100 (-) 1236 WP_163167154.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FSC11_RS12210 (FSC11_12220) ndk 2544223..2544654 (-) 432 WP_004813112.1 nucleoside-diphosphate kinase -
  FSC11_RS12215 (FSC11_12225) iscX 2544766..2544966 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  FSC11_RS12220 (FSC11_12230) - 2545056..2545607 (-) 552 WP_005220946.1 phosphatase PAP2 family protein -
  FSC11_RS12225 (FSC11_12235) - 2545677..2546087 (-) 411 WP_163167156.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30156.40 Da        Isoelectric Point: 9.4172

>NTDB_id=390262 FSC11_RS12200 WP_163167152.1 2542050..2542850(-) (pilF) [Acinetobacter schindleri strain HZE30-1]
MRNLTSKIAFISTVCVSIALSGCQTAEVGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSKLNLDRAETYFKRAISVEPKNAQARNNYGTYLYQLERYNDAIEQFAIAGATLGYDQRYLALENMGRIYLKQGDL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASNMYQQFVRSVGHKNQGARALWIGIRTARAEGDQMGMQVLVNQ
LRALFPESPEYQRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=390262 FSC11_RS12200 WP_163167152.1 2542050..2542850(-) (pilF) [Acinetobacter schindleri strain HZE30-1]
TTGAGAAATCTCACATCTAAAATTGCTTTTATTTCAACAGTATGTGTTTCTATTGCTCTATCTGGATGTCAAACAGCAGA
AGTGGGCAAAAAAGATCCTAAAAAAGCGACCCAGGTGCGTACTCAATTGGCGGTTGAATATCTGAAGACTGGTGATCTGG
ATTCAGCGAAAAGAGCGCTAGATGAGGCGCTTAAAATTAATTCACGTGATGCTCAAGCTAATATGATGATGGGTGTTTTG
TTACAGCAGGAAGGCAGTAAGCTGAACTTGGATCGTGCCGAAACTTATTTTAAGCGCGCGATCTCAGTCGAACCTAAAAA
TGCACAGGCACGTAACAATTATGGCACCTATTTGTATCAGCTTGAACGTTATAATGATGCAATTGAACAGTTTGCAATTG
CAGGCGCGACACTGGGCTATGACCAGCGTTATCTTGCGCTTGAAAATATGGGGCGGATCTACCTGAAGCAAGGTGATCTG
GTGAATGCTGAAAAATCATTCAAACAGGCCCTGCAAGCCAATCGCGATTCTTACATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAATCAGCAGTTCCCGGCAGCGTCGAATATGTATCAACAATTTGTACGTTCAGTGGGACACAAAAACCAGG
GTGCTCGTGCGCTCTGGATCGGCATTCGTACTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTATTGGTCAACCAA
TTGCGCGCATTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-133)

Tetratricopeptide repeat (TPR_2)

(146-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.692

97.744

0.759
  pilF Acinetobacter baylyi ADP1

68.359

96.241

0.658

Multiple sequence alignment