Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FSC12_RS04820 Genome accession   NZ_CP044474
Coordinates   1014429..1015229 (-) Length   266 a.a.
NCBI ID   WP_163168654.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain HZE33-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009429..1020229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC12_RS04800 (FSC12_04820) - 1010474..1011172 (-) 699 WP_004889998.1 tetratricopeptide repeat protein -
  FSC12_RS04805 (FSC12_04825) hisS 1011203..1012498 (-) 1296 WP_163168651.1 histidine--tRNA ligase -
  FSC12_RS04810 (FSC12_04830) ispG 1012502..1013623 (-) 1122 WP_163168652.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FSC12_RS04815 (FSC12_04835) - 1013647..1014438 (-) 792 WP_163168653.1 RodZ domain-containing protein -
  FSC12_RS04820 (FSC12_04840) pilF 1014429..1015229 (-) 801 WP_163168654.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FSC12_RS04825 (FSC12_04845) rlmN 1015244..1016479 (-) 1236 WP_163168655.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FSC12_RS04830 (FSC12_04850) ndk 1016602..1017033 (-) 432 WP_004813112.1 nucleoside-diphosphate kinase -
  FSC12_RS04835 (FSC12_04855) iscX 1017144..1017344 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  FSC12_RS04840 (FSC12_04860) - 1017434..1017985 (-) 552 WP_163168656.1 phosphatase PAP2 family protein -
  FSC12_RS04845 (FSC12_04865) - 1018055..1018465 (-) 411 WP_163168657.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30145.37 Da        Isoelectric Point: 9.4227

>NTDB_id=390195 FSC12_RS04820 WP_163168654.1 1014429..1015229(-) (pilF) [Acinetobacter schindleri strain HZE33-1]
MRNLTSKIAFISTICVSIALSGCQTAEVGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSRLNLDRAETYFKRAISAEPKNAQARNNYGTYLYQLERYNDAIEQFAIAGATLGYDQRYLALENMGRIYLKQGDL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASNMYQQFARSVGQKNQGARALWIGIRIARAEGDQMGMQVLVNQ
LRALFPESPEYQRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=390195 FSC12_RS04820 WP_163168654.1 1014429..1015229(-) (pilF) [Acinetobacter schindleri strain HZE33-1]
TTGAGAAATCTGACATCTAAAATTGCTTTTATTTCAACAATATGTGTTTCTATTGCTCTATCTGGCTGTCAAACGGCAGA
AGTGGGTAAAAAGGATCCTAAAAAAGCGACCCAGGTGCGTACTCAATTGGCGGTTGAATATCTGAAGACTGGTGATCTGG
ATTCAGCGAAAAGAGCGCTAGATGAGGCGCTTAAAATTAATTCACGTGATGCTCAAGCCAATATGATGATGGGTGTTTTG
TTACAGCAGGAAGGTAGTAGGCTGAACTTGGATCGTGCCGAAACTTACTTTAAGCGCGCGATTTCAGCCGAACCTAAAAA
TGCACAGGCACGTAACAATTATGGCACCTATTTGTATCAGCTTGAACGTTATAATGATGCAATTGAACAGTTTGCAATTG
CAGGCGCGACACTGGGCTATGACCAGCGTTATCTTGCGCTTGAAAATATGGGGCGGATCTACCTGAAGCAGGGTGATCTG
GTGAATGCTGAAAAATCATTCAAACAGGCCCTGCAAGCCAATCGCGATTCTTACATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAATCAGCAGTTCCCGGCGGCGTCGAATATGTATCAACAATTTGCACGTTCAGTGGGACAGAAAAACCAGG
GTGCTCGTGCGCTCTGGATCGGCATTCGTATTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTATTGGTCAATCAA
TTACGTGCATTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(150-197)

Tetratricopeptide repeat (TPR_16)

(89-133)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.515

99.248

0.759
  pilF Acinetobacter baylyi ADP1

67.433

98.12

0.662

Multiple sequence alignment