Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FSC10_RS12560 Genome accession   NZ_CP044463
Coordinates   2607110..2607910 (-) Length   266 a.a.
NCBI ID   WP_076753067.1    Uniprot ID   A0A1P8PHG5
Organism   Acinetobacter schindleri strain HZE23-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2602110..2612910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC10_RS12540 (FSC10_12575) - 2603155..2603853 (-) 699 WP_163172127.1 YfgM family protein -
  FSC10_RS12545 (FSC10_12580) hisS 2603884..2605179 (-) 1296 WP_163172130.1 histidine--tRNA ligase -
  FSC10_RS12550 (FSC10_12585) ispG 2605183..2606304 (-) 1122 WP_008303409.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FSC10_RS12555 (FSC10_12590) - 2606328..2607119 (-) 792 WP_163172132.1 helix-turn-helix domain-containing protein -
  FSC10_RS12560 (FSC10_12595) pilF 2607110..2607910 (-) 801 WP_076753067.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FSC10_RS12565 (FSC10_12600) rlmN 2607925..2609160 (-) 1236 WP_005220948.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FSC10_RS12570 (FSC10_12605) ndk 2609283..2609714 (-) 432 WP_004813112.1 nucleoside-diphosphate kinase -
  FSC10_RS12575 (FSC10_12610) iscX 2609825..2610025 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  FSC10_RS12580 (FSC10_12615) - 2610115..2610666 (-) 552 WP_004813115.1 phosphatase PAP2 family protein -
  FSC10_RS12585 (FSC10_12620) - 2610736..2611146 (-) 411 WP_004890015.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30147.35 Da        Isoelectric Point: 9.4227

>NTDB_id=390179 FSC10_RS12560 WP_076753067.1 2607110..2607910(-) (pilF) [Acinetobacter schindleri strain HZE23-1]
MRNLTSKIAFISTVCVSIALSGCQTAEVGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSRLNLDRAETYFKRAISAEPKNAQARNNYGTYLYQLERYNDAIEQFAIAGATLGYDQRYLALENMGRIYLKQGDL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASNMYQQFVRSVGQKNQGARALWIGIRTARAEGDQMGMQVLVNQ
LRALFPESPEYQRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=390179 FSC10_RS12560 WP_076753067.1 2607110..2607910(-) (pilF) [Acinetobacter schindleri strain HZE23-1]
TTGAGAAATCTGACATCTAAAATTGCTTTTATTTCAACAGTATGTGTTTCTATTGCTCTATCTGGCTGTCAAACGGCAGA
AGTGGGTAAAAAGGATCCTAAAAAAGCGACCCAGGTGCGTACTCAATTGGCGGTTGAATATCTGAAGACTGGTGATCTGG
ATTCAGCGAAAAGAGCGCTAGATGAGGCGCTTAAAATTAATTCACGTGATGCTCAAGCCAATATGATGATGGGTGTTTTG
TTACAGCAGGAAGGTAGTAGGCTGAACTTGGATCGTGCCGAAACTTACTTTAAGCGCGCGATTTCAGCCGAACCTAAAAA
TGCACAGGCACGTAACAATTATGGCACCTATTTGTATCAGCTTGAACGTTATAATGATGCAATTGAACAGTTTGCAATTG
CAGGCGCGACACTGGGCTATGACCAGCGTTATCTTGCGCTTGAAAATATGGGGCGGATCTACCTGAAGCAGGGTGATCTG
GTGAATGCTGAAAAATCATTCAAACAGGCCCTGCAAGCCAATCGCGATTCTTACATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAATCAGCAGTTCCCGGCGGCGTCGAATATGTATCAACAATTTGTACGTTCAGTGGGACAGAAAAACCAGG
GTGCTCGTGCGCTCTGGATCGGCATTCGTACTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTATTGGTCAATCAA
TTGCGCGCATTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_2)

(146-176)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8PHG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.692

97.744

0.759
  pilF Acinetobacter baylyi ADP1

69.141

96.241

0.665

Multiple sequence alignment