Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HIR77_RS14645 Genome accession   NZ_CP053102
Coordinates   2765395..2765805 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2760395..2770805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR77_RS14615 (HIR77_14635) - 2760482..2760622 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  HIR77_RS14620 (HIR77_14640) yqeF 2760928..2761659 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  HIR77_RS14625 (HIR77_14645) cwlH 2761911..2762663 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  HIR77_RS14630 (HIR77_14650) yqeD 2762850..2763476 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  HIR77_RS14635 (HIR77_14655) gnd 2763495..2764388 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  HIR77_RS14640 (HIR77_14660) yqeB 2764640..2765362 (+) 723 WP_010886572.1 hypothetical protein -
  HIR77_RS14645 (HIR77_14665) nucA/comI 2765395..2765805 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  HIR77_RS14650 (HIR77_14670) - 2766001..2766348 (+) 348 Protein_2856 sigma-70 family RNA polymerase sigma factor -
  HIR77_RS14655 (HIR77_14675) spoIVCA 2766379..2767839 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  HIR77_RS22825 - 2767797..2767975 (-) 179 Protein_2858 hypothetical protein -
  HIR77_RS14660 (HIR77_14680) arsC 2768330..2768749 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  HIR77_RS14665 (HIR77_14685) acr3 2768761..2769801 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  HIR77_RS14670 (HIR77_14690) arsK 2769824..2770264 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  HIR77_RS14675 (HIR77_14695) arsR 2770325..2770642 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=390049 HIR77_RS14645 WP_009967785.1 2765395..2765805(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=390049 HIR77_RS14645 WP_009967785.1 2765395..2765805(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment