Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MPQ_RS07350 Genome accession   NC_014733
Coordinates   1534291..1535649 (-) Length   452 a.a.
NCBI ID   WP_041362159.1    Uniprot ID   -
Organism   Methylovorus sp. MP688     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1529291..1540649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPQ_RS07320 (MPQ_1495) hpnE 1530009..1531358 (+) 1350 WP_013442276.1 hydroxysqualene dehydroxylase HpnE -
  MPQ_RS07325 (MPQ_1496) - 1531355..1531750 (+) 396 WP_015830119.1 phage holin family protein -
  MPQ_RS07330 (MPQ_1497) - 1531762..1532508 (+) 747 WP_013442278.1 YciK family oxidoreductase -
  MPQ_RS07335 (MPQ_1498) - 1532543..1532785 (-) 243 WP_013442279.1 hypothetical protein -
  MPQ_RS07340 (MPQ_1499) - 1532968..1533597 (+) 630 WP_013442280.1 DUF2238 domain-containing protein -
  MPQ_RS07345 (MPQ_1500) - 1533626..1534270 (-) 645 WP_013442281.1 HAD family hydrolase -
  MPQ_RS07350 (MPQ_1501) radA 1534291..1535649 (-) 1359 WP_041362159.1 DNA repair protein RadA Machinery gene
  MPQ_RS07355 (MPQ_1502) alr 1535642..1536712 (-) 1071 WP_013442283.1 alanine racemase -
  MPQ_RS07360 (MPQ_1503) dnaB 1536725..1538107 (-) 1383 WP_013442284.1 replicative DNA helicase -
  MPQ_RS07365 (MPQ_1504) - 1538160..1539329 (-) 1170 WP_013442285.1 cystathionine beta-lyase -
  MPQ_RS07370 (MPQ_1505) rplI 1539446..1539895 (-) 450 WP_013442286.1 50S ribosomal protein L9 -
  MPQ_RS07375 (MPQ_1506) rpsR 1539907..1540134 (-) 228 WP_013442287.1 30S ribosomal protein S18 -
  MPQ_RS07380 (MPQ_1507) priB 1540154..1540465 (-) 312 WP_013442288.1 primosomal replication protein N -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48169.78 Da        Isoelectric Point: 7.4498

>NTDB_id=39001 MPQ_RS07350 WP_041362159.1 1534291..1535649(-) (radA) [Methylovorus sp. MP688]
MAKAKTVYTCTECGASSPKWQGQCPSCMAWNTLVESIAEAPTANRYAALAQTAGLQKLAEVEAAEVPRQPSGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQTLCHVGHLRKAIYVSGEESAQQIAMRARRLGLDAGPIDLLAEIQLEKILATLQT
HKPDIAVIDSIQTIYSEALQSAPGSVAQVRECSAQLTRAAKQLGITVILVGHVTKEGSLAGPRVLEHIVDTVLYFEGDPN
SSFRLIRAFKNRFGAVNELGVFAMTEKGLREVSNPSALFLSHHAEQVAGSCITVTQEGSRPLLVEIQALVDEAHAPNPKR
LCVGLEQNRLAMLLAVLHRHAGVACFDQDVFINAVGGVRISEPAVDLAVLLAIVSSLKNKPLHNKLIVFGEVGLAGEVRP
VQRGQERLKEAAKLGFTHAIVPKANAPKQAIPDLTVIAVERLDQALQKIKEL

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=39001 MPQ_RS07350 WP_041362159.1 1534291..1535649(-) (radA) [Methylovorus sp. MP688]
ATGGCTAAGGCAAAAACCGTTTACACCTGCACCGAGTGCGGGGCATCCTCACCCAAATGGCAAGGACAGTGCCCCAGTTG
CATGGCATGGAACACGCTGGTCGAGAGCATAGCGGAAGCGCCGACGGCCAACCGCTATGCAGCATTGGCGCAGACTGCTG
GCTTGCAAAAGCTGGCGGAAGTGGAGGCAGCCGAGGTGCCGCGCCAGCCTTCTGGCATCGGCGAGTTTGATCGTGTGCTG
GGCGGCGGCCTGGTGGCAGGCGGGGTCGTGCTGATCGGGGGCGACCCGGGCATAGGCAAGTCCACCTTGCTGTTGCAGAC
ACTGTGCCACGTTGGCCATCTGCGTAAAGCCATCTACGTTAGCGGTGAGGAGTCTGCGCAGCAGATTGCCATGCGTGCCC
GCCGTCTGGGGCTGGATGCTGGCCCGATTGATCTCCTGGCCGAAATTCAGCTGGAAAAAATTCTGGCGACCCTGCAGACG
CACAAGCCGGATATTGCCGTCATCGACTCCATCCAGACCATTTACTCCGAGGCCTTGCAGTCTGCCCCTGGCTCGGTGGC
ACAGGTGCGTGAATGCTCAGCCCAGTTAACACGTGCCGCCAAGCAACTCGGTATCACGGTGATCCTGGTAGGCCATGTGA
CCAAGGAAGGCTCGCTCGCCGGGCCACGCGTGTTGGAGCATATTGTCGATACCGTGCTGTATTTCGAGGGCGACCCCAAC
TCCAGTTTCCGCCTGATTCGCGCGTTCAAAAACCGCTTTGGAGCGGTCAACGAGCTGGGTGTGTTTGCCATGACAGAGAA
GGGCTTGCGCGAAGTTAGCAATCCATCGGCGCTGTTTCTTTCCCATCATGCCGAGCAAGTGGCGGGCTCTTGTATTACGG
TTACCCAGGAAGGCTCACGGCCTTTGCTGGTCGAGATACAGGCCCTGGTCGATGAGGCGCATGCACCCAACCCCAAGCGC
CTTTGCGTGGGCCTTGAGCAGAATCGGCTGGCCATGTTGCTGGCGGTATTGCATCGCCATGCGGGTGTCGCATGCTTTGA
CCAGGATGTGTTTATCAATGCGGTGGGTGGCGTGCGGATCAGCGAGCCTGCAGTCGATCTTGCGGTATTGCTGGCGATAG
TATCTTCTTTGAAAAACAAGCCACTGCATAACAAACTGATTGTATTTGGTGAGGTTGGGCTGGCGGGAGAGGTGAGGCCG
GTACAACGTGGTCAGGAGCGCCTGAAGGAAGCGGCCAAGCTGGGCTTCACCCATGCCATCGTGCCCAAGGCAAATGCCCC
CAAGCAAGCCATTCCGGATTTGACGGTTATCGCTGTCGAACGCCTGGATCAAGCCTTGCAGAAAATTAAAGAACTATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-217)

Subunit ChlI of Mg-chelatase (ChlI)

(341-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.448

100

0.496
  radA Streptococcus pneumoniae Rx1

48.115

99.779

0.48
  radA Streptococcus pneumoniae D39

48.115

99.779

0.48
  radA Streptococcus pneumoniae R6

48.115

99.779

0.48
  radA Streptococcus pneumoniae TIGR4

48.115

99.779

0.48
  radA Streptococcus mitis SK321

48.115

99.779

0.48
  radA Streptococcus mitis NCTC 12261

47.894

99.779

0.478

Multiple sequence alignment