Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   F7V86_RS05050 Genome accession   NZ_CP044444
Coordinates   966664..967809 (+) Length   381 a.a.
NCBI ID   WP_014419129.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain KC41     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 961664..972809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7V86_RS05030 fnr 962138..962854 (-) 717 WP_017418713.1 fumarate/nitrate reduction transcriptional regulator Fnr -
  F7V86_RS05035 narK 962952..964142 (-) 1191 WP_017418712.1 nitrate transporter NarK -
  F7V86_RS05040 argS 964277..965947 (-) 1671 WP_025649385.1 arginine--tRNA ligase -
  F7V86_RS05045 - 965944..966372 (-) 429 WP_014419128.1 DUF1934 domain-containing protein -
  F7V86_RS05050 rapF 966664..967809 (+) 1146 WP_014419129.1 tetratricopeptide repeat protein Regulator
  F7V86_RS05055 - 967793..967912 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  F7V86_RS05060 - 968110..968313 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  F7V86_RS05065 - 968327..968530 (+) 204 WP_007407641.1 hypothetical protein -
  F7V86_RS05070 - 968565..968750 (+) 186 WP_014419130.1 DUF4177 domain-containing protein -
  F7V86_RS05075 - 968937..969218 (+) 282 WP_065180304.1 hypothetical protein -
  F7V86_RS05080 - 969262..969774 (+) 513 WP_014419132.1 stage II sporulation protein M -
  F7V86_RS05090 - 970365..970583 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  F7V86_RS05095 speB 970736..971608 (-) 873 WP_003151054.1 agmatinase -
  F7V86_RS05100 speE 971668..972498 (-) 831 WP_017418706.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45581.65 Da        Isoelectric Point: 4.5797

>NTDB_id=389966 F7V86_RS05050 WP_014419129.1 966664..967809(+) (rapF) [Bacillus amyloliquefaciens strain KC41]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=389966 F7V86_RS05050 WP_014419129.1 966664..967809(+) (rapF) [Bacillus amyloliquefaciens strain KC41]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCTATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.215

100

0.782
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment