Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MPQ_RS01055 Genome accession   NC_014733
Coordinates   228575..230029 (-) Length   484 a.a.
NCBI ID   WP_013441028.1    Uniprot ID   -
Organism   Methylovorus sp. MP688     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 223575..235029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPQ_RS01025 (MPQ_0207) ptsP 223835..225589 (-) 1755 WP_013441022.1 phosphoenolpyruvate--protein phosphotransferase -
  MPQ_RS01030 (MPQ_0208) - 225597..225866 (-) 270 WP_013441023.1 HPr family phosphocarrier protein -
  MPQ_RS01035 (MPQ_0209) - 225859..226266 (-) 408 WP_013441024.1 PTS sugar transporter subunit IIA -
  MPQ_RS01040 (MPQ_0210) rapZ 226317..227153 (-) 837 WP_041362502.1 RNase adapter RapZ -
  MPQ_RS01045 (MPQ_0211) hprK 227170..228135 (-) 966 WP_013441026.1 HPr(Ser) kinase/phosphatase -
  MPQ_RS01050 (MPQ_0212) hpf 228218..228547 (-) 330 WP_013441027.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MPQ_RS01055 (MPQ_0213) rpoN/rpoN1 228575..230029 (-) 1455 WP_013441028.1 RNA polymerase factor sigma-54 Machinery gene
  MPQ_RS01060 (MPQ_0214) lptB 230054..230776 (-) 723 WP_013441029.1 LPS export ABC transporter ATP-binding protein -
  MPQ_RS01065 (MPQ_0215) lptA 230833..231390 (-) 558 WP_013441030.1 lipopolysaccharide transport periplasmic protein LptA -
  MPQ_RS01070 (MPQ_0216) lptC 231371..232126 (-) 756 WP_015829205.1 LPS export ABC transporter periplasmic protein LptC -
  MPQ_RS01075 (MPQ_0217) - 232136..232660 (-) 525 WP_013441032.1 HAD family hydrolase -
  MPQ_RS01080 (MPQ_0218) - 232662..233660 (-) 999 WP_013441033.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 54606.28 Da        Isoelectric Point: 5.0235

>NTDB_id=38990 MPQ_RS01055 WP_013441028.1 228575..230029(-) (rpoN/rpoN1) [Methylovorus sp. MP688]
MKQSLQLKFSQHLALTPQLQQSIRLLQLSTQELNQELETLLQANPLLERADKIEEPLEIPDNFIAAPVAPEPSVAATPEE
APRQDEFGGDDYFEFGSSSGSNRWEDSSSGNDDDNDFSYQEAVSLSLRDHLLGQLKLLPLSERDQTLSMLLVDTITDDGY
LEQSLEELAEMLPPELEIDMLELQTALKHIQNLDPPGIGARNLAECLSLQLRALPPDTPYVDLAIKVAETHLPLLAARDY
AKLKKLLACDDDVLREVQQLVLQLNPRPGGEFSKITSDHYIQHEIVVKKIKGVWLAHLNDEVVPKLRINQLYAGILKRNR
ESSSQYLMSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFEHGEVAMRPLVLREIADELGLHESTVSRVTTRKYMLT
PRGVYELKYFFGSHVATESGGACSATAIRALIKQMVSEENPKKPLSDNQITDILGKQGIVVARRTIAKYRESLQIPAANQ
RRSL

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=38990 MPQ_RS01055 WP_013441028.1 228575..230029(-) (rpoN/rpoN1) [Methylovorus sp. MP688]
ATGAAGCAAAGCTTACAACTCAAATTTTCGCAACACCTGGCGCTGACTCCGCAACTGCAGCAGTCCATCCGTTTGTTGCA
GCTATCCACGCAGGAGCTCAATCAGGAGCTGGAAACCCTACTGCAGGCGAATCCATTGCTGGAACGCGCTGACAAGATCG
AAGAACCGCTGGAAATCCCCGACAACTTCATTGCGGCGCCAGTAGCCCCTGAGCCCAGCGTGGCCGCCACACCGGAGGAA
GCTCCCCGCCAGGATGAATTTGGTGGCGATGACTATTTTGAATTCGGCAGCAGCAGCGGCAGCAACCGCTGGGAAGACAG
CAGCTCCGGCAATGACGATGACAATGATTTCAGCTACCAGGAAGCGGTCAGCCTCAGCCTGCGCGATCATCTGCTAGGTC
AGCTCAAACTGTTGCCACTGTCCGAGCGCGACCAGACCTTGTCCATGCTGCTGGTCGATACCATTACCGACGACGGTTAT
CTGGAGCAGTCGCTGGAAGAGCTGGCCGAGATGCTGCCACCGGAGCTCGAAATCGACATGCTGGAGTTGCAGACGGCGCT
CAAGCATATCCAGAACCTGGACCCACCCGGCATAGGCGCGCGCAATCTGGCAGAATGCCTATCCTTGCAATTGCGCGCCT
TGCCACCAGATACGCCTTATGTGGATCTCGCCATCAAGGTTGCCGAAACGCACTTGCCCCTGCTGGCAGCCCGCGATTAC
GCAAAACTGAAAAAATTGCTGGCCTGCGACGATGATGTATTGCGCGAAGTGCAACAGCTGGTGCTGCAACTCAATCCCCG
CCCAGGTGGTGAATTCAGCAAAATCACGTCTGATCATTACATTCAGCATGAAATCGTGGTCAAGAAAATCAAAGGTGTCT
GGCTGGCCCATCTGAACGACGAAGTCGTGCCCAAGCTGCGCATCAACCAGCTGTATGCCGGCATCCTCAAGCGCAACCGC
GAAAGCTCGAGCCAATACCTGATGAGCCAGATGCAGGAAGCCAAGTGGATGATCAAGAACATCCAGCAGCGCTTCTCCAC
CATCCTGCGCGTTTCTCAGGCGATTGTGGATCGCCAGCGCAATTTCTTTGAGCACGGCGAGGTCGCCATGCGCCCCCTGG
TGCTGCGCGAGATTGCCGATGAGCTGGGCCTGCACGAATCCACGGTGTCACGCGTTACCACGCGCAAGTACATGCTCACC
CCACGCGGAGTTTACGAGCTTAAATATTTCTTTGGCAGCCATGTGGCTACCGAATCCGGGGGCGCTTGTTCCGCCACTGC
CATACGCGCCCTGATCAAACAAATGGTCAGCGAAGAGAACCCGAAAAAGCCGCTGTCGGATAACCAGATCACGGATATCC
TGGGTAAACAGGGCATCGTGGTTGCTCGTCGCACCATTGCCAAATACCGCGAGTCTTTGCAGATACCCGCGGCCAACCAG
CGCAGATCGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(123-312)

Sigma-54, DNA binding domain (Sigma54_DBD)

(325-482)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.36

100

0.558

Multiple sequence alignment