Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HKW69_RS20400 Genome accession   NZ_CP053080
Coordinates   4235826..4236266 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain HB37     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4230826..4241266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW69_RS20380 (HKW69_20395) aroP 4231826..4233196 (+) 1371 WP_000399019.1 aromatic amino acid transporter AroP -
  HKW69_RS20385 (HKW69_20400) ampE 4233239..4234093 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  HKW69_RS20390 (HKW69_20405) ampD 4234090..4234641 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HKW69_RS20395 (HKW69_20410) nadC 4234729..4235622 (+) 894 WP_001135161.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HKW69_RS20400 (HKW69_20415) pilA 4235826..4236266 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  HKW69_RS20405 (HKW69_20420) pilB 4236276..4237661 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  HKW69_RS20410 (HKW69_20425) hofC 4237651..4238853 (+) 1203 WP_170172014.1 protein transport protein HofC -
  HKW69_RS20415 (HKW69_20430) guaC 4238888..4239931 (-) 1044 WP_001217338.1 GMP reductase -
  HKW69_RS26940 - 4240087..4240131 (-) 45 WP_120795372.1 protein YacM -
  HKW69_RS20420 (HKW69_20435) coaE 4240156..4240776 (+) 621 WP_001269522.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=389751 HKW69_RS20400 WP_000360914.1 4235826..4236266(+) (pilA) [Escherichia coli strain HB37]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=389751 HKW69_RS20400 WP_000360914.1 4235826..4236266(+) (pilA) [Escherichia coli strain HB37]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363