Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BACIH_RS01545 Genome accession   NZ_CP044360
Coordinates   287147..287584 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain V167     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 282147..292584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BACIH_RS01520 (BACIH_0289) - 283166..284302 (+) 1137 WP_012116766.1 zinc-dependent alcohol dehydrogenase -
  BACIH_RS01525 (BACIH_0290) - 284317..284751 (+) 435 WP_012116767.1 RDD family protein -
  BACIH_RS01530 (BACIH_0291) - 284869..285147 (+) 279 WP_263612330.1 YckD family protein -
  BACIH_RS01535 (BACIH_0292) - 285251..286687 (+) 1437 WP_012116768.1 glycoside hydrolase family 1 protein -
  BACIH_RS01540 (BACIH_0293) nin/comJ 286728..287126 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  BACIH_RS01545 (BACIH_0294) nucA/comI 287147..287584 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BACIH_RS01550 (BACIH_0295) hxlB 287943..288500 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  BACIH_RS01555 (BACIH_0296) hxlA 288497..289132 (-) 636 WP_012116769.1 3-hexulose-6-phosphate synthase -
  BACIH_RS01560 (BACIH_0297) - 289364..289726 (+) 363 WP_012116770.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=389641 BACIH_RS01545 WP_003156588.1 287147..287584(-) (nucA/comI) [Bacillus amyloliquefaciens strain V167]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=389641 BACIH_RS01545 WP_003156588.1 287147..287584(-) (nucA/comI) [Bacillus amyloliquefaciens strain V167]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment