Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F5976_RS11215 Genome accession   NZ_CP044228
Coordinates   2128071..2129435 (+) Length   454 a.a.
NCBI ID   WP_005686111.1    Uniprot ID   A0A2A5L5Z5
Organism   Lacticaseibacillus rhamnosus strain hsryfm 1301     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2123071..2134435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F5976_RS11185 (F5976_11185) - 2123447..2124793 (-) 1347 WP_005714538.1 C1 family peptidase -
  F5976_RS11190 (F5976_11190) - 2124938..2126257 (-) 1320 WP_005709712.1 aminopeptidase C -
  F5976_RS11200 (F5976_11200) - 2126552..2126842 (-) 291 WP_005686109.1 GNAT family N-acetyltransferase -
  F5976_RS11210 (F5976_11210) - 2127512..2128051 (+) 540 WP_005686110.1 dUTP diphosphatase -
  F5976_RS11215 (F5976_11215) radA 2128071..2129435 (+) 1365 WP_005686111.1 DNA repair protein RadA Machinery gene
  F5976_RS11220 (F5976_11220) - 2129456..2130571 (+) 1116 WP_005686113.1 PIN/TRAM domain-containing protein -
  F5976_RS16215 (F5976_11225) - 2130663..2130755 (-) 93 Protein_2017 Rgg/GadR/MutR family transcriptional regulator -
  F5976_RS11230 (F5976_11230) - 2130884..2132200 (+) 1317 WP_005686114.1 MucBP domain-containing protein -
  F5976_RS11235 (F5976_11235) - 2132393..2132779 (+) 387 WP_005686116.1 GntR family transcriptional regulator -
  F5976_RS11240 (F5976_11240) - 2132776..2133792 (+) 1017 Protein_2020 DUF5808 domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49157.58 Da        Isoelectric Point: 8.3128

>NTDB_id=388943 F5976_RS11215 WP_005686111.1 2128071..2129435(+) (radA) [Lacticaseibacillus rhamnosus strain hsryfm 1301]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSTKSAPRQTRTGSKVKPTRMDEVTVTKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIENVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWRAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=388943 F5976_RS11215 WP_005686111.1 2128071..2129435(+) (radA) [Lacticaseibacillus rhamnosus strain hsryfm 1301]
ATGGCGAAAGCAAAAACACAATATGTATGTCAAAATTGCGGTTATATTTCGGCGACTTATTTAGGCCGTTGTCCAAACTG
CGGCGGGTGGAATACGCTCGTCGAAGAAACCGTTAGTTCCACTAAATCGGCGCCGCGACAAACGCGCACCGGCAGCAAGG
TTAAGCCCACACGCATGGACGAGGTCACGGTGACCAAAGAAACCCGGGTTAAAACAGGCTTGGCCGAGTTGAATCGCGTG
CTTGGTGGCGGTGTGGTTCCGGGCTCGTTGGTTCTGATTGGCGGTGATCCCGGGATTGGCAAATCAACCTTGCTGCTGCA
GGTGTCTGGTCAGCTAGCTAGCACAGGTGGTACGGTGTTGTACGTTTCAGGTGAAGAAAGCGCCAGTCAGATTAAAATGC
GGGCAGGGCGGCTAGGTGTTGCCAACTCCGGCATGTATTTATATCCCGAAACCGACATGCCCAGCATCGAAAATGTGATT
GACCAGATGCAACCGGATTATGTGGTGATTGACTCGGTTCAAACCATGAATGTACCGGACATGAATTCGGCAGTCGGCTC
GGTTGCCCAGATTCGGGAAGTCACCGCCGAATTGATGCGAATTGCCAAGTCAAAAGGTGTGACGATTTTTATCGTTGGTC
ACGTGACGAAGGAAGGCGCTATTGCCGGGCCCAAGATTCTTGAGCATATGGTGGACACCGTGCTTTATTTTGAGGGCGAT
ATGCATCATACCTATCGCATCTTAAGGTCGGTCAAGAATCGGTTCGGCTCTACTAACGAAATCGGTATTTTCGAAATGCA
TCAAAATGGCTTACAGGAAGTTGCCAATCCCTCGGAAATCTTCCTGGAAGAACGGCTGGCCGGTGCAACCGGCTCGGCGG
TGGTGGTTTCGATGGAAGGTACCCGTCCGATTCTGGTTGAAATCCAGGCCCTCATCAGCCCGACCATGTATGGCAATGCC
AAACGCACGAGCAGCGGCTTGGATCACAACCGCGTGAGCTTGATCATGGCGGTGCTGGAAAAACGTGCCAATTTGATGTT
GCAAAATCAGGACGCTTACTTGAAAGCAACAGGGGGCGTTAAGCTGGATGAGCCGGCGATTGACTTGGCAATGGCAGTGG
CAATTGCCTCGTCATACCGCGACAAGGAAATCCCGCCGACTGATTGTTTTGTCGGTGAAATTGGCTTAACCGGTGAAGTG
CGGCGGGTGAATCGCATTGAAGACCGGGTGAAAGAGGCGGCTAAGTTAGGGTTTAAACGAATTTTTGTGCCGCGAAATAA
CCTGCAAGGCTGGCGTGCGCCTAAAGATATTCAGGTTATTGGCGTCACAAGCATTGCCGAAGCGTTGCATAAGGTTTTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5L5Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

64.912

100

0.652

Multiple sequence alignment