Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   F4V27_RS00650 Genome accession   NZ_CP044167
Coordinates   130468..131421 (+) Length   317 a.a.
NCBI ID   WP_057100841.1    Uniprot ID   -
Organism   Campylobacter jejuni strain AR-0415     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 114507..144407 130468..131421 within 0


Gene organization within MGE regions


Location: 114507..144407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F4V27_RS00570 pglI 114507..115436 (-) 930 WP_057100522.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  F4V27_RS00575 pglH 115429..116508 (-) 1080 WP_074469282.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  F4V27_RS00580 pglK 116505..118198 (-) 1694 Protein_115 ABC-type lipopolysaccharide transporter PglK -
  F4V27_RS00585 galE 118192..119178 (-) 987 WP_002893768.1 UDP-glucose 4-epimerase GalE -
  F4V27_RS00590 - 119237..120031 (-) 795 WP_057100907.1 3'-5' exonuclease -
  F4V27_RS00595 waaC 120097..121125 (+) 1029 WP_002893766.1 lipopolysaccharide heptosyltransferase I -
  F4V27_RS00600 htrB 121115..122002 (+) 888 WP_057100908.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  F4V27_RS00605 - 121999..123546 (+) 1548 WP_057100909.1 glycosyltransferase -
  F4V27_RS08855 - 123550..124019 (+) 470 Protein_121 glycosyltransferase family A protein -
  F4V27_RS00615 - 124384..125004 (-) 621 Protein_122 glycosyltransferase family 2 protein -
  F4V27_RS00620 - 125093..125970 (+) 878 Protein_123 alpha-2,3-sialyltransferase -
  F4V27_RS00625 neuB 125971..127011 (+) 1041 WP_057100837.1 N-acetylneuraminate synthase -
  F4V27_RS00630 neuC 127008..128126 (+) 1119 WP_057100838.1 UDP-N-acetylglucosamine 2-epimerase -
  F4V27_RS00635 - 128123..128788 (+) 666 WP_150968482.1 acylneuraminate cytidylyltransferase family protein -
  F4V27_RS08860 - 128776..128949 (+) 174 WP_002896733.1 hypothetical protein -
  F4V27_RS08925 - 128991..129605 (+) 615 WP_283151597.1 CatB-related O-acetyltransferase -
  F4V27_RS00645 - 129597..130409 (-) 813 WP_057100840.1 glycosyltransferase family 2 protein -
  F4V27_RS00650 waaF 130468..131421 (+) 954 WP_057100841.1 lipopolysaccharide heptosyltransferase II Regulator
  F4V27_RS00655 - 131428..132186 (-) 759 WP_057100842.1 glycosyltransferase family 25 protein -
  F4V27_RS00660 gmhA 132186..132746 (-) 561 WP_057100843.1 D-sedoheptulose 7-phosphate isomerase -
  F4V27_RS00665 rfaE1 132743..134128 (-) 1386 WP_002922733.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  F4V27_RS00670 rfaD 134121..135074 (-) 954 WP_057100844.1 ADP-glyceromanno-heptose 6-epimerase -
  F4V27_RS00675 gmhB 135075..135635 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  F4V27_RS00680 - 135717..136019 (+) 303 WP_002852762.1 cytochrome c -
  F4V27_RS00685 ccoS 136042..136248 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  F4V27_RS00690 - 136245..138602 (-) 2358 WP_079745947.1 heavy metal translocating P-type ATPase -
  F4V27_RS00695 rho 138711..140009 (+) 1299 WP_057100448.1 transcription termination factor Rho -
  F4V27_RS00700 dnaX 140013..141542 (+) 1530 WP_057100447.1 DNA polymerase III subunit gamma/tau -
  F4V27_RS00705 - 141620..141769 (-) 150 WP_079745946.1 DUF2165 family protein -
  F4V27_RS00710 - 141795..141902 (-) 108 WP_191904444.1 DUF2165 family protein -
  F4V27_RS08930 - 141908..142042 (-) 135 WP_284675261.1 hypothetical protein -
  F4V27_RS00715 copA 142114..144213 (-) 2100 WP_057100446.1 copper-translocating P-type ATPase CopA -
  F4V27_RS00720 - 144213..144407 (-) 195 WP_002929627.1 cation transporter -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 36472.12 Da        Isoelectric Point: 10.3105

>NTDB_id=388459 F4V27_RS00650 WP_057100841.1 130468..131421(+) (waaF) [Campylobacter jejuni strain AR-0415]
MKIFIHLPTWLGDAVMASPALYTIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQTRYKQALSLRKKLGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEIIIEVSKQLLFLN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=388459 F4V27_RS00650 WP_057100841.1 130468..131421(+) (waaF) [Campylobacter jejuni strain AR-0415]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACACCATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAAACCCGCTACAAACAAGCCCTATCTTTACGCAAAAAACTTGGTAAAATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAGCAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACCCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCACTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGTATGAAAGATTTAAAGCCTGAAATCATCATAGAAGTAAGTAAACAATTGCTATTTCTAAATTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(95-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

94.586

99.054

0.937

Multiple sequence alignment