Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   F4V31_RS08535 Genome accession   NZ_CP044162
Coordinates   1634956..1635915 (+) Length   319 a.a.
NCBI ID   WP_002927706.1    Uniprot ID   -
Organism   Campylobacter jejuni strain AR-0419     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1614625..1639958 1634956..1635915 within 0


Gene organization within MGE regions


Location: 1614625..1639958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F4V31_RS08420 pglI 1614625..1615554 (-) 930 WP_002852770.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  F4V31_RS08425 pglH 1615547..1616626 (-) 1080 WP_010891911.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  F4V31_RS08430 pglK 1616623..1618329 (-) 1707 WP_150967489.1 ABC-type lipopolysaccharide transporter PglK -
  F4V31_RS08435 galE 1618310..1619296 (-) 987 WP_002852683.1 UDP-glucose 4-epimerase GalE -
  F4V31_RS08440 - 1619354..1620148 (-) 795 WP_002868613.1 3'-5' exonuclease -
  F4V31_RS08445 waaC 1620215..1621243 (+) 1029 WP_002927649.1 lipopolysaccharide heptosyltransferase I -
  F4V31_RS08450 htrB 1621233..1622111 (+) 879 WP_002927653.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  F4V31_RS08455 - 1622108..1622809 (+) 702 WP_002902664.1 glycosyltransferase family 2 protein -
  F4V31_RS08460 rfbA 1622820..1623698 (+) 879 WP_002927657.1 glucose-1-phosphate thymidylyltransferase RfbA -
  F4V31_RS08465 rfbB 1623698..1624729 (+) 1032 WP_002927659.1 dTDP-glucose 4,6-dehydratase -
  F4V31_RS08470 wlaRA 1624732..1625172 (+) 441 WP_079865040.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  F4V31_RS08475 wlaRB 1625169..1625573 (+) 405 WP_002927665.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  F4V31_RS08480 - 1625563..1626030 (+) 468 WP_002927676.1 acyltransferase -
  F4V31_RS08485 - 1626027..1626554 (+) 528 WP_002876185.1 GNAT family N-acetyltransferase -
  F4V31_RS08495 - 1626851..1627927 (+) 1077 WP_002876186.1 glycosyltransferase family A protein -
  F4V31_RS08500 wlaRG 1627924..1629009 (+) 1086 WP_002876187.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  F4V31_RS08505 - 1629041..1629961 (-) 921 WP_251814079.1 DUF2972 domain-containing protein -
  F4V31_RS08510 - 1630290..1631312 (-) 1023 WP_150967484.1 hypothetical protein -
  F4V31_RS08515 - 1631383..1632156 (+) 774 WP_002866160.1 glycosyltransferase family 25 protein -
  F4V31_RS08520 wlaTB 1632149..1633105 (+) 957 WP_002860237.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  F4V31_RS08525 wlaTC 1633102..1634061 (+) 960 WP_002927702.1 class E lipooligosaccharide biosynthesis protein WlaTC -
  F4V31_RS08530 - 1634064..1634897 (-) 834 WP_002927704.1 glycosyltransferase family 2 protein -
  F4V31_RS08535 waaF 1634956..1635915 (+) 960 WP_002927706.1 lipopolysaccharide heptosyltransferase II Regulator
  F4V31_RS08540 gmhA 1635896..1636456 (-) 561 WP_002787744.1 D-sedoheptulose 7-phosphate isomerase -
  F4V31_RS08545 rfaE1 1636453..1637838 (-) 1386 WP_002927708.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  F4V31_RS08550 rfaD 1637831..1638784 (-) 954 WP_002873533.1 ADP-glyceromanno-heptose 6-epimerase -
  F4V31_RS08555 gmhB 1638785..1639345 (-) 561 WP_002927709.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  F4V31_RS08560 - 1639427..1639729 (+) 303 WP_002852762.1 cytochrome c -
  F4V31_RS08565 ccoS 1639752..1639958 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36906.42 Da        Isoelectric Point: 10.3311

>NTDB_id=388452 F4V31_RS08535 WP_002927706.1 1634956..1635915(+) (waaF) [Campylobacter jejuni strain AR-0419]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELDKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDVSYFAKVALNFSQSHEILIFGTGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENARWVHLNLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=388452 F4V31_RS08535 WP_002927706.1 1634956..1635915(+) (waaF) [Campylobacter jejuni strain AR-0419]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGACAAAATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAGCAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGTGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTACAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAGATGGGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(95-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.417

100

0.934

Multiple sequence alignment