Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HIR76_RS13970 Genome accession   NZ_CP051860
Coordinates   2676498..2676908 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2671498..2681908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR76_RS13940 (HIR76_13665) - 2671585..2671725 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  HIR76_RS13945 (HIR76_13660) yqeF 2672031..2672762 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  HIR76_RS13950 (HIR76_13655) cwlH 2673014..2673766 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  HIR76_RS13955 (HIR76_13650) yqeD 2673953..2674579 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  HIR76_RS13960 (HIR76_13645) gnd 2674598..2675491 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  HIR76_RS13965 (HIR76_13640) yqeB 2675743..2676465 (+) 723 WP_010886572.1 hypothetical protein -
  HIR76_RS13970 (HIR76_13635) nucA/comI 2676498..2676908 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  HIR76_RS13975 (HIR76_13630) - 2677104..2677451 (+) 348 Protein_2720 sigma-70 family RNA polymerase sigma factor -
  HIR76_RS13980 (HIR76_13625) spoIVCA 2677482..2678942 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  HIR76_RS22145 - 2678900..2679078 (-) 179 Protein_2722 hypothetical protein -
  HIR76_RS13985 (HIR76_13620) arsC 2679433..2679852 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  HIR76_RS13990 (HIR76_13615) acr3 2679864..2680904 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  HIR76_RS13995 (HIR76_13610) arsK 2680927..2681367 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  HIR76_RS14000 (HIR76_13605) arsR 2681428..2681745 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=388323 HIR76_RS13970 WP_009967785.1 2676498..2676908(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=388323 HIR76_RS13970 WP_009967785.1 2676498..2676908(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529