Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   HIR76_RS02855 Genome accession   NZ_CP051860
Coordinates   547314..548489 (+) Length   391 a.a.
NCBI ID   WP_009966637.1    Uniprot ID   P96649
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 542314..553489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR76_RS02835 (HIR76_02665) cwlT 544030..545019 (+) 990 WP_009966633.1 bifunctional lytic transglycosylase/C40 family peptidase -
  HIR76_RS02840 (HIR76_02660) - 545034..545540 (+) 507 WP_009966634.1 hypothetical protein -
  HIR76_RS02845 (HIR76_02655) conJ 545603..545983 (+) 381 WP_009969670.1 DUF4467 domain-containing protein -
  HIR76_RS02850 (HIR76_02650) spbK 546174..546974 (-) 801 WP_009966636.1 toll/interleukin-1 receptor domain-containing protein -
  HIR76_RS02855 (HIR76_02645) rapF 547314..548489 (+) 1176 WP_009966637.1 response regulator aspartate phosphatase RapI Regulator
  HIR76_RS02860 (HIR76_02640) phrI 548446..548565 (+) 120 WP_010886415.1 phosphatase RapI inhibitor PhrI -
  HIR76_RS02865 (HIR76_02635) yddM 548718..549659 (+) 942 WP_010886416.1 helicase -
  HIR76_RS02870 (HIR76_02630) yddN 550248..551267 (-) 1020 WP_003234259.1 LLM class flavin-dependent oxidoreductase -
  HIR76_RS02875 (HIR76_02625) lrpA 551527..551937 (+) 411 WP_003246601.1 transcriptional regulator LrpA -
  HIR76_RS02880 (HIR76_02620) lrpB 552060..552509 (-) 450 WP_003246612.1 transcriptional regulator LrpB -
  HIR76_RS02885 (HIR76_02615) yddQ 552624..553166 (+) 543 WP_003234256.1 cysteine hydrolase family protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 46566.15 Da        Isoelectric Point: 4.9688

>NTDB_id=388239 HIR76_RS02855 WP_009966637.1 547314..548489(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=388239 HIR76_RS02855 WP_009966637.1 547314..548489(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(10-70)

Tetratricopeptide repeat (TPR_12)

(199-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4I1A

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

38.525

93.606

0.361
  rapC Bacillus subtilis subsp. subtilis str. 168

38.108

94.629

0.361

Multiple sequence alignment