Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AXYL_RS00700 Genome accession   NC_014640
Coordinates   148344..149789 (+) Length   481 a.a.
NCBI ID   WP_013390904.1    Uniprot ID   E3HPC3
Organism   Achromobacter xylosoxidans A8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143344..154789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXYL_RS00685 (AXYL_00137) - 144014..145342 (+) 1329 WP_013390901.1 amidase -
  AXYL_RS00690 (AXYL_00138) leuA 145482..147194 (-) 1713 WP_013390902.1 2-isopropylmalate synthase -
  AXYL_RS00695 (AXYL_00139) - 147716..148345 (-) 630 WP_013390903.1 hypothetical protein -
  AXYL_RS00700 (AXYL_00140) rpoN/rpoN1 148344..149789 (+) 1446 WP_013390904.1 RNA polymerase factor sigma-54 Machinery gene
  AXYL_RS00705 (AXYL_00141) - 149923..150204 (+) 282 WP_013390905.1 bacterioferritin-associated ferredoxin -
  AXYL_RS00710 (AXYL_00142) - 150235..151092 (-) 858 WP_013390906.1 LysR substrate-binding domain-containing protein -
  AXYL_RS00715 (AXYL_00143) - 151223..152137 (+) 915 WP_013390907.1 DMT family transporter -
  AXYL_RS00720 (AXYL_00144) bfr 152504..152980 (+) 477 WP_013390908.1 bacterioferritin -
  AXYL_RS00725 (AXYL_00145) - 153061..153747 (-) 687 WP_193353239.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52295.59 Da        Isoelectric Point: 4.8998

>NTDB_id=38821 AXYL_RS00700 WP_013390904.1 148344..149789(+) (rpoN/rpoN1) [Achromobacter xylosoxidans A8]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLERQDEAPSAETPAESDRESSVQDDEP
AAERETPVDEMPGSGGVYPDEDSSRPEAARPDTLREHLLGQLMLTRAAPRDVALAGLLIDELDENGYLGSPLEEILGWLP
AELEPDIDELKAALSLLQSFDPPGIGARDMAECLLLQLRNPDLGQLPEAADAAVLACARQICAQHLALLATGNAARLCAA
VGCDDATFRSAHSLILRLEPRPGRAWTVPAADYAVPDVIVRKARGGWQVTLNSAAVPKLQINGLYAQMLGNQKESAHAGL
HSQLQQARWMIRNVEQRFDTILRVAQAIVQHQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAVQTFIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=38821 AXYL_RS00700 WP_013390904.1 148344..149789(+) (rpoN/rpoN1) [Achromobacter xylosoxidans A8]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCTGGTCAGCATTTGACGCTGACGCCACAACTGCA
GCAATCGATCCGATTGTTGCAGCTGTCCACGCTCGATCTCGAAGCCGAGATCTCGCAAGTGCTTGCGGAGAACCCGCTGC
TGGAGCGGCAGGACGAAGCGCCGTCGGCCGAGACGCCGGCGGAGTCCGACCGCGAGTCCTCCGTGCAGGACGACGAACCC
GCCGCCGAACGCGAGACCCCGGTGGACGAAATGCCGGGCTCGGGCGGGGTCTACCCCGACGAAGATTCCAGCCGGCCCGA
GGCCGCAAGGCCGGACACGCTGCGCGAGCACCTGCTGGGCCAACTGATGCTGACGCGCGCTGCGCCGCGTGACGTCGCGT
TGGCGGGCCTGCTGATCGACGAGCTGGACGAGAACGGCTACCTGGGATCGCCGCTGGAAGAAATCCTGGGCTGGTTGCCG
GCCGAGCTGGAGCCGGACATCGACGAGCTGAAGGCGGCCCTGTCGCTGCTGCAATCGTTCGACCCGCCCGGCATTGGCGC
CCGCGACATGGCCGAATGCCTGTTGTTGCAGCTGCGCAACCCCGATCTGGGCCAATTGCCCGAAGCGGCGGATGCCGCGG
TGCTGGCCTGTGCGCGGCAGATCTGCGCGCAACACCTCGCGCTGCTGGCGACAGGCAACGCTGCGCGCCTGTGCGCGGCG
GTGGGCTGCGACGACGCCACGTTCCGTTCGGCGCATTCCCTGATCCTGCGGCTGGAGCCGCGTCCGGGGCGCGCCTGGAC
CGTGCCCGCGGCCGACTACGCCGTGCCCGACGTCATCGTGCGCAAGGCCCGTGGCGGCTGGCAGGTCACCCTCAACAGCG
CCGCCGTGCCCAAGCTGCAGATCAATGGGTTGTACGCCCAGATGTTGGGCAACCAGAAGGAGTCCGCGCATGCGGGGCTG
CACTCGCAGTTGCAGCAGGCCCGCTGGATGATCCGCAACGTGGAGCAGCGCTTTGACACCATCCTGCGGGTGGCCCAGGC
CATCGTGCAACACCAGACGGCTTTCTTCAGCCAAGGACCTGCCGCCATGCGGCCGCTCATCCTGAAGGACATCGCCGGCG
AACTGGGCTTGCACGAATCCACTATTTCACGGGCCACAACGCAGAAGTACATGCTCACTCCGTTTGGCACATTGGAGCTC
AAGCGCTTCTTCGGCACGGGCGTGTCGACGGACGGCGGGGATGCCACCTCGGCCACGGCGGTGCAGACCTTCATCCGCCA
GATGGTGGCCGATGAGAACCGGGCCAAGCCTTTGTCCGACAGCCAGATCATGCAAAAGCTGGCCGATCAGGGGATAGTCA
TCGCCCGAAGGACGGTGGCAAAGTATCGAGAAGCGCTGCGGATTGCCCCGGCGGCGTTGCGCAAGGCGCAGGCGGCCGCC
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E3HPC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.228

100

0.509

Multiple sequence alignment