Detailed information NTG0388134

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: HHJ20_RS01225
• Genome accession: NZ_CP051849
• Coordinates: 257218..258201 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli strain SCU-101
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 252218..263201

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HHJ20_RS01205 (HHJ20_01200)	dppA	253362..254969 (+)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-
HHJ20_RS01210 (HHJ20_01205)	-	255031..255258 (-)	228	WP_000198577.1	hypothetical protein	-
HHJ20_RS01215 (HHJ20_01210)	dppB	255276..256295 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
HHJ20_RS01220 (HHJ20_01215)	dppC	256305..257207 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
HHJ20_RS01225 (HHJ20_01220)	amiE	257218..258201 (+)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
HHJ20_RS01230 (HHJ20_01225)	dppF	258198..259202 (+)	1005	WP_000103580.1	dipeptide ABC transporter ATP-binding subunit DppF	-
HHJ20_RS01235 (HHJ20_01230)	yhjV	259232..260503 (-)	1272	WP_001298005.1	aromatic amino acid transport family protein	-
HHJ20_RS01240 (HHJ20_01235)	-	260979..261086 (+)	108	WP_000170738.1	type I toxin-antitoxin system toxin Ldr family protein	-
HHJ20_RS01245 (HHJ20_01240)	-	261462..261569 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=388134 HHJ20_RS01225 WP_001196477.1 257218..258201(+) (amiE) [Escherichia coli strain SCU-101]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=388134 HHJ20_RS01225 WP_001196477.1 257218..258201(+) (amiE) [Escherichia coli strain SCU-101]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGTGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCAATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAAGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGACCAAAACTGCTGATTGCCGATGAACCGACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404