Detailed information NTG0388053

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: HHJ30_RS00860
• Genome accession: NZ_CP051765
• Coordinates: 184643..185626 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain SCU-113
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 179643..190626

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HHJ30_RS00845 (HHJ30_00845)	dppA	180943..182550 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
HHJ30_RS00850 (HHJ30_00850)	dppB	182701..183720 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
HHJ30_RS00855 (HHJ30_00855)	dppC	183730..184632 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
HHJ30_RS00860 (HHJ30_00860)	amiE	184643..185626 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
HHJ30_RS00865 (HHJ30_00865)	dppF	185623..186627 (+)	1005	WP_000107030.1	dipeptide ABC transporter ATP-binding subunit DppF	-
HHJ30_RS00870 (HHJ30_00870)	yhjV	186657..187928 (-)	1272	WP_001402620.1	aromatic amino acid transport family protein	-
HHJ30_RS00875 (HHJ30_00875)	-	188404..188511 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
HHJ30_RS00880 (HHJ30_00880)	-	188887..188994 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
HHJ30_RS00885 (HHJ30_00885)	-	189370..189477 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=388053 HHJ30_RS00860 WP_001196486.1 184643..185626(+) (amiE) [Escherichia coli strain SCU-113]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=388053 HHJ30_RS00860 WP_001196486.1 184643..185626(+) (amiE) [Escherichia coli strain SCU-113]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCTGGCGGTATGAGTCAG
CGTGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGTTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGTAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGATGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404