Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HHJ43_RS02650 Genome accession   NZ_CP051751
Coordinates   570104..571537 (-) Length   477 a.a.
NCBI ID   WP_096986734.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 565104..576537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ43_RS02615 (HHJ43_02620) elbB 565796..566449 (+) 654 WP_001397829.1 isoprenoid biosynthesis glyoxalase ElbB -
  HHJ43_RS02620 (HHJ43_02625) mtgA 566446..567174 (+) 729 WP_000047098.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HHJ43_RS02625 (HHJ43_02630) yrbL 567171..567803 (-) 633 WP_000620399.1 PhoP regulatory network protein YrbL -
  HHJ43_RS02630 (HHJ43_02635) npr 568016..568288 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  HHJ43_RS02635 (HHJ43_02640) rapZ 568285..569139 (-) 855 WP_000243741.1 RNase adapter RapZ -
  HHJ43_RS02640 (HHJ43_02645) ptsN 569185..569676 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HHJ43_RS02645 (HHJ43_02650) hpf 569794..570081 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  HHJ43_RS02650 (HHJ43_02655) rpoN/rpoN1 570104..571537 (-) 1434 WP_096986734.1 RNA polymerase factor sigma-54 Machinery gene
  HHJ43_RS02655 (HHJ43_02660) lptB 571585..572310 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  HHJ43_RS02660 (HHJ43_02665) lptA 572317..572874 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HHJ43_RS02665 (HHJ43_02670) lptC 572843..573418 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  HHJ43_RS02670 (HHJ43_02675) kdsC 573415..573981 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HHJ43_RS02675 (HHJ43_02680) kdsD 574002..574988 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  HHJ43_RS02680 (HHJ43_02685) yrbG 575002..575979 (-) 978 WP_096986735.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54023.82 Da        Isoelectric Point: 4.3639

>NTDB_id=387899 HHJ43_RS02650 WP_096986734.1 570104..571537(-) (rpoN/rpoN1) [Escherichia coli strain SCU-301]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTESGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=387899 HHJ43_RS02650 WP_096986734.1 570104..571537(-) (rpoN/rpoN1) [Escherichia coli strain SCU-301]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACTGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
TTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGAGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment