Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ28_RS18340 Genome accession   NZ_CP051727
Coordinates   3843450..3843890 (+) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3838450..3848890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ28_RS18320 (HHJ28_18320) aroP 3839447..3840820 (+) 1374 WP_074424235.1 aromatic amino acid transporter AroP -
  HHJ28_RS18325 (HHJ28_18325) ampE 3840863..3841717 (-) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  HHJ28_RS18330 (HHJ28_18330) ampD 3841714..3842265 (-) 552 WP_000923733.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ28_RS18335 (HHJ28_18335) nadC 3842353..3843246 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ28_RS18340 (HHJ28_18340) pilA 3843450..3843890 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ28_RS18345 (HHJ28_18345) pilB 3843900..3845285 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  HHJ28_RS18350 (HHJ28_18350) hofC 3845275..3846477 (+) 1203 WP_000157289.1 protein transport protein HofC -
  HHJ28_RS18355 (HHJ28_18355) guaC 3846512..3847555 (-) 1044 WP_001217338.1 GMP reductase -
  HHJ28_RS24295 - 3847711..3847755 (-) 45 WP_120795372.1 protein YacM -
  HHJ28_RS18360 (HHJ28_18360) coaE 3847780..3848400 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=387523 HHJ28_RS18340 WP_000360913.1 3843450..3843890(+) (pilA) [Escherichia coli strain SCU-111]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=387523 HHJ28_RS18340 WP_000360913.1 3843450..3843890(+) (pilA) [Escherichia coli strain SCU-111]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACTACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363