Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FOB72_RS12975 Genome accession   NZ_CP044065
Coordinates   2836514..2837998 (+) Length   494 a.a.
NCBI ID   WP_150372891.1    Uniprot ID   A0A5P2H577
Organism   Cupriavidus pauculus strain FDAARGOS_664     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2831514..2842998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB72_RS12955 (FOB72_12950) - 2833185..2834168 (+) 984 WP_150372887.1 SIS domain-containing protein -
  FOB72_RS12960 (FOB72_12955) lptC 2834185..2834814 (+) 630 WP_150372888.1 LPS export ABC transporter periplasmic protein LptC -
  FOB72_RS12965 (FOB72_12960) lptA 2834891..2835559 (+) 669 WP_150372889.1 lipopolysaccharide transport periplasmic protein LptA -
  FOB72_RS12970 (FOB72_12965) lptB 2835600..2836391 (+) 792 WP_150372890.1 LPS export ABC transporter ATP-binding protein -
  FOB72_RS12975 (FOB72_12970) rpoN/rpoN1 2836514..2837998 (+) 1485 WP_150372891.1 RNA polymerase factor sigma-54 Machinery gene
  FOB72_RS12980 (FOB72_12975) hpf 2838048..2838401 (+) 354 WP_109582176.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  FOB72_RS12985 (FOB72_12980) ptsN 2838708..2839163 (+) 456 WP_150372892.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FOB72_RS12990 (FOB72_12985) hprK 2839265..2840236 (+) 972 WP_109582178.1 HPr(Ser) kinase/phosphatase -
  FOB72_RS12995 (FOB72_12990) - 2840354..2840677 (+) 324 WP_150372893.1 PsiF family protein -
  FOB72_RS13000 (FOB72_12995) rapZ 2840739..2841635 (+) 897 WP_150372894.1 RNase adapter RapZ -
  FOB72_RS13005 (FOB72_13000) - 2841704..2842378 (+) 675 WP_150372895.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 54905.19 Da        Isoelectric Point: 4.8732

>NTDB_id=387355 FOB72_RS12975 WP_150372891.1 2836514..2837998(+) (rpoN/rpoN1) [Cupriavidus pauculus strain FDAARGOS_664]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQSAPAPAPAEPQ
GNGEERAEGAGGSDEEGFGGDNTSEDYGNDWSLDDFARRPQGDEDEKTPMQLRDAEPTLREHLMEQVAPLKISLRDKGLA
VFLIESLDDDGYLGATLEEIFSELPEELEFEIEEVQSMLTLLQSFDPPGVGARNAAECLTLQLRRISHPQRDLASSIVNH
HLELLAVRDYTRLKKALQVDEIALKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGGGWIAQLNPDVMPRLRINDM
YAQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTIS
RVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPKNPLSDSRIAELLGEQGFVVARRTVAKYR
EALKIPAVNLRKTL

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=387355 FOB72_RS12975 WP_150372891.1 2836514..2837998(+) (rpoN/rpoN1) [Cupriavidus pauculus strain FDAARGOS_664]
ATGAAACCGTCGCTTCAGCTTCGCCTCTCCCAGCACCTTGCCCTTACCCCCCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCGACGTTGGAGCTCCAGCAGGAAGTGGAGCAGGCTCTGACGGAGAATCCCCTGCTCGAGCGCGAAAACGACTGGA
TCGAGAGCCCGCTGCGCGTGGCCGCCGATGGCTCCGTCAACGTGCAGAGCGCCCCCGCGCCCGCCCCGGCCGAGCCGCAG
GGCAACGGCGAGGAGCGCGCGGAAGGCGCGGGCGGCAGCGACGAGGAAGGCTTCGGCGGCGATAACACCAGCGAGGACTA
CGGCAACGACTGGAGCCTCGACGATTTCGCGCGCCGTCCGCAGGGCGACGAGGACGAGAAGACGCCGATGCAGTTGCGCG
ACGCGGAACCCACGCTGCGCGAGCATCTGATGGAACAGGTCGCGCCGCTGAAGATTTCCCTGCGCGACAAGGGCCTGGCG
GTGTTCCTGATCGAGTCGCTCGACGACGATGGCTATCTCGGCGCCACCCTCGAAGAAATTTTCTCGGAACTGCCCGAGGA
ACTCGAGTTCGAAATAGAGGAAGTGCAGTCGATGCTCACGCTGCTGCAGAGCTTCGATCCGCCCGGCGTGGGCGCGCGCA
ATGCGGCGGAATGTCTCACGCTGCAGCTGCGCCGCATCTCGCATCCGCAACGCGATCTTGCATCGTCGATCGTCAACCAC
CATCTGGAGTTACTGGCGGTGCGTGATTACACGCGCCTCAAGAAAGCGCTCCAGGTGGACGAAATAGCATTGAAGGCCGC
GCACGATCTGATCCGCTCGCTAGCACCGTATCCCGGGCACGCCTATAGCAGGCCCGAGGCCGATTTCGTGGTGCCCGACG
TGTTCGTGCGCAAGAGCGGAGGTGGCTGGATTGCCCAGCTGAATCCCGATGTGATGCCGCGATTACGCATCAACGACATG
TACGCGCAGATCCTGCGCGGCGCGAAGGGCGAATCGGGCACTGCGGGGCTGCAGCAGAAGCTTCAGGAGGCGCGGTGGCT
CATCAAGAACATACAGCAGCGCTTCGATACGATCCTGCGTGTGGCGCAGGCTATCGTCGAACGGCAGAAGAACTTTTTCA
CCCATGGGGAAATCGCGATGCGCCCCTTGGTTTTGAGGGAGATAGCCGATACACTCGGTTTACATGAGTCCACGATTTCG
CGCGTGACCACGAACAAGTACATGGCCACGCCGATGGGGACTTTCGAGTTGAAGTACTTCTTCGGGAGCCACGTGTCCAC
CGAAACGGGTGGCGCAGCTTCATCCACGGCAATCCGCGCGCTGATCAAGCAACTCATAGGAGCCGAAGACCCCAAGAATC
CCCTTTCCGACAGCCGCATCGCCGAACTGTTGGGCGAGCAGGGCTTTGTGGTCGCGCGGCGCACCGTCGCCAAATACCGC
GAAGCGCTCAAGATCCCGGCAGTGAATCTCCGCAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-321)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P2H577

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

77.888

100

0.791

Multiple sequence alignment