Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOB75_RS15410 Genome accession   NZ_CP044062
Coordinates   3029293..3030333 (+) Length   346 a.a.
NCBI ID   WP_045608015.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FDAARGOS_667     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3024293..3035333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB75_RS15380 (FOB75_15380) - 3025434..3026036 (-) 603 WP_005482459.1 XTP/dITP diphosphatase -
  FOB75_RS15385 (FOB75_15385) - 3026154..3026585 (-) 432 WP_045545354.1 DUF4426 domain-containing protein -
  FOB75_RS15390 (FOB75_15390) yggU 3026713..3027003 (-) 291 WP_005482461.1 DUF167 family protein YggU -
  FOB75_RS15395 (FOB75_15395) - 3027003..3027560 (-) 558 WP_005482440.1 YggT family protein -
  FOB75_RS15400 (FOB75_15400) proC 3027613..3028431 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  FOB75_RS15405 (FOB75_15405) - 3028551..3029264 (-) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOB75_RS15410 (FOB75_15410) pilT 3029293..3030333 (+) 1041 WP_045608015.1 type IV pilus twitching motility protein PilT Machinery gene
  FOB75_RS15415 (FOB75_15415) pilU 3030364..3031470 (+) 1107 WP_005461714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOB75_RS15420 (FOB75_15420) ruvX 3031532..3031957 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  FOB75_RS15425 (FOB75_15425) - 3032006..3032569 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  FOB75_RS15430 (FOB75_15430) gshB 3032683..3033633 (-) 951 WP_031851951.1 glutathione synthase -
  FOB75_RS15435 (FOB75_15435) rsmE 3033648..3034379 (-) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FOB75_RS15440 (FOB75_15440) endA 3034504..3035199 (-) 696 WP_005493878.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38548.02 Da        Isoelectric Point: 6.5037

>NTDB_id=387306 FOB75_RS15410 WP_045608015.1 3029293..3030333(+) (pilT) [Vibrio parahaemolyticus strain FDAARGOS_667]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEDLEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=387306 FOB75_RS15410 WP_045608015.1 3029293..3030333(+) (pilT) [Vibrio parahaemolyticus strain FDAARGOS_667]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTGTTCCGTACCATCCCAAGTAGCATCCCGACTCTGGA
AGATCTAGAAGCGCCAGAGATCTTCAAAAAAATCGCGAATGCTGAAAAAGGTTTGGTTTTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACAACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATCCTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAAGTTCACCGTGATACCCATAGCTTCCAAAA
CGCGCTACGCAGCGCATTGCGTGAAGACCCGGATGTGATCCTTGTCGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CGTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGTACGTTGCACACCAGTTCAGCAGCAAAAACCATCGACCGTATC
ATCGATGTGTTCCCAGGTAGCGACAAAGACATGGTGCGTTCCATGTTGTCTGAATCCCTTCGTTCGGTTATCGCGCAAAA
ACTGTTGAAACGTAATGGCGGTGGTCGTATCGCTTGTCACGAAATCATGATGGCAACGCCCGCGATTCGTAACTTGATTC
GCGAAGACAAAGTCGCGCAGATGTACTCAATCATTCAAACGGGTGCCGCGCATGGAATGCAAACCATGGAACAAAATGCC
CGTCAATTGATGGCGCAAGGTATGGTTTCGCGCGAAGAAGTCGACAGCAAGATTGGGCTTGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

99.711

0.87
  pilT Vibrio cholerae strain A1552

87.246

99.711

0.87
  pilT Acinetobacter baumannii D1279779

73.03

95.376

0.697
  pilT Acinetobacter baumannii strain A118

73.03

95.376

0.697
  pilT Acinetobacter nosocomialis M2

72.727

95.376

0.694
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.399

0.679
  pilT Acinetobacter baylyi ADP1

71.212

95.376

0.679
  pilT Pseudomonas aeruginosa PAK

69.436

97.399

0.676
  pilT Legionella pneumophila strain Lp02

68.58

95.665

0.656
  pilT Legionella pneumophila strain ERS1305867

68.58

95.665

0.656
  pilT Neisseria meningitidis 8013

65.465

96.243

0.63
  pilT Neisseria gonorrhoeae MS11

65.165

96.243

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.532

0.399
  pilU Acinetobacter baylyi ADP1

39.688

92.486

0.367

Multiple sequence alignment