Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FOB40_RS13025 Genome accession   NZ_CP044060
Coordinates   2866566..2868005 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain FDAARGOS_632     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2861566..2873005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB40_RS12995 (FOB40_12995) dagF 2861834..2862574 (+) 741 WP_150389847.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  FOB40_RS13000 (FOB40_13000) - 2862682..2864586 (+) 1905 WP_150389849.1 transcription antiterminator -
  FOB40_RS13005 (FOB40_13005) npr 2864604..2864876 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  FOB40_RS13010 (FOB40_13010) rapZ 2864897..2865763 (-) 867 WP_040068818.1 RNase adapter RapZ -
  FOB40_RS13015 (FOB40_13015) ptsN 2865809..2866255 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FOB40_RS13020 (FOB40_13020) hpf 2866258..2866545 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  FOB40_RS13025 (FOB40_13025) rpoN/rpoN1 2866566..2868005 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  FOB40_RS13030 (FOB40_13030) lptB 2868071..2868796 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  FOB40_RS13035 (FOB40_13035) lptA 2868800..2869327 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  FOB40_RS13040 (FOB40_13040) lptC 2869314..2869874 (-) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  FOB40_RS13045 (FOB40_13045) kdsC 2869871..2870425 (-) 555 WP_047438734.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FOB40_RS13050 (FOB40_13050) - 2870425..2871420 (-) 996 WP_058057518.1 KpsF/GutQ family sugar-phosphate isomerase -
  FOB40_RS13055 (FOB40_13055) mlaF 2871628..2872428 (+) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=387263 FOB40_RS13025 WP_005336331.1 2866566..2868005(-) (rpoN/rpoN1) [Aeromonas veronii strain FDAARGOS_632]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=387263 FOB40_RS13025 WP_005336331.1 2866566..2868005(-) (rpoN/rpoN1) [Aeromonas veronii strain FDAARGOS_632]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCA
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGTGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAAGGAGAGACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATAATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGTGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTTCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATTTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCACGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCCATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment