Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FOB31_RS13995 Genome accession   NZ_CP044054
Coordinates   2323414..2324919 (-) Length   501 a.a.
NCBI ID   WP_006398255.1    Uniprot ID   -
Organism   Burkholderia multivorans strain FDAARGOS_623     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2318414..2329919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB31_RS13970 (FOB31_13965) - 2319442..2320077 (-) 636 WP_053296824.1 LON peptidase substrate-binding domain-containing protein -
  FOB31_RS13975 (FOB31_13970) rapZ 2320105..2321013 (-) 909 WP_006409809.1 RNase adapter RapZ -
  FOB31_RS13980 (FOB31_13975) hprK 2321092..2322060 (-) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  FOB31_RS13985 (FOB31_13980) - 2322189..2322701 (-) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  FOB31_RS13990 (FOB31_13985) raiA 2322896..2323255 (-) 360 WP_006398254.1 ribosome-associated translation inhibitor RaiA -
  FOB31_RS13995 (FOB31_13990) rpoN/rpoN1 2323414..2324919 (-) 1506 WP_006398255.1 RNA polymerase factor sigma-54 Machinery gene
  FOB31_RS14000 (FOB31_13995) lptB 2325072..2325848 (-) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  FOB31_RS14005 (FOB31_14000) lptA 2325845..2326510 (-) 666 WP_006398257.1 lipopolysaccharide transport periplasmic protein LptA -
  FOB31_RS14010 (FOB31_14005) lptC 2326531..2327145 (-) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  FOB31_RS14015 (FOB31_14010) - 2327149..2327685 (-) 537 WP_006398259.1 HAD family hydrolase -
  FOB31_RS14020 (FOB31_14015) kdsD 2327685..2328668 (-) 984 WP_006406620.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54746.94 Da        Isoelectric Point: 4.8740

>NTDB_id=387207 FOB31_RS13995 WP_006398255.1 2323414..2324919(-) (rpoN/rpoN1) [Burkholderia multivorans strain FDAARGOS_623]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=387207 FOB31_RS13995 WP_006398255.1 2323414..2324919(-) (rpoN/rpoN1) [Burkholderia multivorans strain FDAARGOS_623]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGTCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCAGCCGGCGCCGACGAATACGACGGCTACGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCAAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCATCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCGCTGCGCGACGCACACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAGTCGGCCAGGGCTGGCAGGCCGAGCTCAATCCTGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCACACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695

Multiple sequence alignment