Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ35_RS18535 Genome accession   NZ_CP051714
Coordinates   3879471..3879911 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain SCU-122     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3874471..3884911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ35_RS18515 (HHJ35_18525) aroP 3875471..3876841 (+) 1371 WP_033547925.1 aromatic amino acid transporter AroP -
  HHJ35_RS18520 (HHJ35_18530) ampE 3876884..3877738 (-) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  HHJ35_RS18525 (HHJ35_18535) ampD 3877735..3878286 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ35_RS18530 (HHJ35_18540) nadC 3878374..3879267 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ35_RS18535 (HHJ35_18545) pilA 3879471..3879911 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ35_RS18540 (HHJ35_18550) pilB 3879921..3881306 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  HHJ35_RS18545 (HHJ35_18555) hofC 3881296..3882498 (+) 1203 WP_000157254.1 protein transport protein HofC -
  HHJ35_RS18550 (HHJ35_18560) guaC 3882533..3883576 (-) 1044 WP_001217338.1 GMP reductase -
  HHJ35_RS24180 - 3883732..3883776 (-) 45 WP_120795372.1 protein YacM -
  HHJ35_RS18555 (HHJ35_18565) coaE 3883801..3884421 (+) 621 WP_001269535.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=387182 HHJ35_RS18535 WP_000360914.1 3879471..3879911(+) (pilA) [Escherichia coli strain SCU-122]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=387182 HHJ35_RS18535 WP_000360914.1 3879471..3879911(+) (pilA) [Escherichia coli strain SCU-122]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363