Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ELI_RS16395 Genome accession   NC_014624
Coordinates   3408045..3409406 (-) Length   453 a.a.
NCBI ID   WP_013381921.1    Uniprot ID   E3GG74
Organism   Eubacterium callanderi     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3403045..3414406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELI_RS21470 (ELI_3652) scfA 3403196..3403336 (-) 141 WP_013381915.1 six-cysteine ranthipeptide SCIFF -
  ELI_RS21070 (ELI_3653) yajC 3403362..3403805 (-) 444 WP_013381916.1 preprotein translocase subunit YajC -
  ELI_RS16375 (ELI_3654) tgt 3403824..3404933 (-) 1110 WP_013381917.1 tRNA guanosine(34) transglycosylase Tgt -
  ELI_RS16380 (ELI_3655) queA 3404946..3405980 (-) 1035 WP_013381918.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  ELI_RS16385 (ELI_3656) ruvB 3405991..3407019 (-) 1029 WP_013381919.1 Holliday junction branch migration DNA helicase RuvB -
  ELI_RS16390 (ELI_3657) ruvA 3407031..3407642 (-) 612 WP_013381920.1 Holliday junction branch migration protein RuvA -
  ELI_RS16395 (ELI_3658) radA 3408045..3409406 (-) 1362 WP_013381921.1 DNA repair protein RadA Machinery gene
  ELI_RS16400 (ELI_3659) - 3409417..3409902 (-) 486 WP_013381922.1 VanZ family protein -
  ELI_RS16405 (ELI_3660) - 3409971..3411020 (-) 1050 WP_013381923.1 PTS transporter subunit IIC -
  ELI_RS16410 (ELI_3661) - 3411288..3411929 (-) 642 WP_013381924.1 acetolactate decarboxylase -
  ELI_RS16415 (ELI_3662) - 3411939..3412859 (-) 921 WP_013381925.1 CobW family GTP-binding protein -
  ELI_RS16420 (ELI_3663) - 3412918..3414267 (-) 1350 WP_013381926.1 uroporphyrinogen decarboxylase family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50478.83 Da        Isoelectric Point: 8.3489

>NTDB_id=38716 ELI_RS16395 WP_013381921.1 3408045..3409406(-) (radA) [Eubacterium callanderi]
MAKIKKKFVCQNCGYSTPKWMGRCTECGEWNSFVEELDMPTEQGKKNTLERAVYTKPKRIEEIIPQKEDRFKTKNKELDR
VLGGGIVPGGVILLGGDPGIGKSTILLQTTENLGSQGLKILYISGEESEQQLKMRAVRMKVKSQNIFFLSEINVPYIVDT
ILHEKPDLVIIDSIQTMYSPTITSAPGSVSQIRENANALMQIAKKDNISMILVGHVTKEGNIAGPRVLEHMVDTVLYFEG
EKYHTYRILRGVKNRFGSTNEIGIFEMAQDGLHEVANPSEMMLSSRPKNTCGSVVVPCMEGTRPLLIELQGLVSQTSFGN
PRRMATGMDYNRMVLLLAIMEKRLGLQLQNLDAYINVVGGMKIDEPALDLAVVSVLYSSFRNFEIPEDMMIIGEVGLTGE
VRNIQHIEKRLKEGAKLGFKRCIMPKGNARGLENTGLELLPVDNISKALNILK

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=38716 ELI_RS16395 WP_013381921.1 3408045..3409406(-) (radA) [Eubacterium callanderi]
ATGGCGAAAATAAAAAAGAAATTTGTGTGCCAGAACTGCGGGTACAGCACGCCTAAATGGATGGGGCGCTGTACAGAATG
TGGCGAATGGAACTCCTTTGTTGAGGAGCTGGATATGCCCACTGAGCAGGGAAAGAAGAATACCCTTGAACGGGCGGTGT
ACACAAAACCCAAGCGTATTGAAGAAATTATTCCTCAGAAAGAGGATCGTTTTAAAACGAAGAATAAGGAGCTCGACCGC
GTCCTCGGCGGCGGCATTGTGCCTGGAGGCGTTATCCTTCTAGGAGGTGACCCGGGAATTGGAAAGTCAACCATTTTGCT
GCAGACGACGGAAAATCTTGGAAGCCAGGGGCTTAAAATCCTGTATATTTCAGGTGAGGAGTCCGAGCAGCAGCTCAAAA
TGCGCGCAGTCCGGATGAAGGTGAAATCCCAGAATATTTTTTTTCTGTCTGAGATTAACGTGCCCTATATCGTAGATACT
ATTTTGCATGAAAAGCCCGATCTGGTCATTATTGACTCGATTCAGACAATGTACAGTCCTACAATTACCTCAGCGCCCGG
AAGCGTCAGCCAGATAAGGGAGAACGCTAACGCGTTGATGCAGATTGCCAAAAAAGACAATATTTCGATGATTCTGGTCG
GACATGTGACCAAGGAAGGCAATATTGCCGGTCCCCGTGTGTTAGAACATATGGTTGATACGGTTTTGTATTTTGAGGGT
GAAAAGTACCATACCTACCGGATACTCAGAGGGGTCAAAAACCGTTTTGGCTCAACCAATGAGATCGGTATTTTCGAAAT
GGCGCAGGATGGCCTGCACGAGGTGGCAAATCCCTCTGAAATGATGCTGTCGAGCCGGCCGAAAAATACCTGTGGCTCTG
TGGTAGTGCCCTGCATGGAGGGGACACGTCCGCTTTTAATCGAGCTTCAGGGGCTCGTATCACAGACAAGCTTCGGTAAC
CCAAGGCGTATGGCCACCGGAATGGACTATAACCGTATGGTGCTGCTCCTGGCCATTATGGAAAAAAGGCTAGGATTACA
GCTTCAAAATCTTGATGCGTATATCAATGTGGTCGGGGGTATGAAGATAGATGAGCCGGCTCTTGATCTGGCCGTTGTGT
CTGTGCTATACTCAAGCTTCAGGAATTTTGAAATTCCAGAGGATATGATGATTATCGGCGAGGTAGGCCTGACTGGTGAG
GTCAGAAATATACAGCATATTGAAAAGCGCCTTAAAGAAGGCGCGAAGCTTGGCTTTAAGCGCTGTATCATGCCAAAGGG
CAATGCCCGGGGCCTGGAAAATACAGGGCTGGAGCTTCTGCCAGTCGATAATATATCTAAGGCCTTAAATATATTGAAAT
AG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(62-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(355-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E3GG74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

55.752

99.779

0.556
  radA Streptococcus mitis NCTC 12261

52.546

95.364

0.501
  radA Streptococcus pneumoniae Rx1

52.546

95.364

0.501
  radA Streptococcus pneumoniae D39

52.546

95.364

0.501
  radA Streptococcus pneumoniae R6

52.546

95.364

0.501
  radA Streptococcus pneumoniae TIGR4

52.546

95.364

0.501
  radA Streptococcus mitis SK321

52.546

95.364

0.501

Multiple sequence alignment