Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ40_RS19660 Genome accession   NZ_CP051698
Coordinates   4120760..4121200 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SCU-152     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4115760..4126200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ40_RS19640 (HHJ40_19645) coaE 4116250..4116870 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  HHJ40_RS23315 - 4116895..4116939 (+) 45 WP_120795372.1 protein YacM -
  HHJ40_RS19645 (HHJ40_19650) guaC 4117095..4118138 (+) 1044 WP_001217338.1 GMP reductase -
  HHJ40_RS19650 (HHJ40_19655) hofC 4118173..4119375 (-) 1203 WP_000157236.1 protein transport protein HofC -
  HHJ40_RS19655 (HHJ40_19660) pilB 4119365..4120750 (-) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  HHJ40_RS19660 (HHJ40_19665) pilA 4120760..4121200 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ40_RS19665 (HHJ40_19670) nadC 4121403..4122296 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ40_RS19670 (HHJ40_19675) ampD 4122384..4122935 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ40_RS19675 (HHJ40_19680) ampE 4122932..4123786 (+) 855 WP_000171978.1 beta-lactamase regulator AmpE -
  HHJ40_RS19680 (HHJ40_19685) aroP 4123829..4125202 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=386847 HHJ40_RS19660 WP_000360904.1 4120760..4121200(-) (pilA) [Escherichia coli strain SCU-152]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=386847 HHJ40_RS19660 WP_000360904.1 4120760..4121200(-) (pilA) [Escherichia coli strain SCU-152]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment