Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F2A31_RS02655 Genome accession   NZ_CP043909
Coordinates   549963..550763 (+) Length   266 a.a.
NCBI ID   WP_026056343.1    Uniprot ID   A0AAW4JFL0
Organism   Acinetobacter suaedae strain C16S1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544963..555763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F2A31_RS02630 (F2A31_02630) - 545872..547188 (+) 1317 WP_171490547.1 glycosyltransferase family 1 protein -
  F2A31_RS02635 (F2A31_02635) - 547212..547763 (+) 552 WP_150025043.1 phosphatase PAP2 family protein -
  F2A31_RS02640 (F2A31_02640) iscX 547848..548048 (+) 201 WP_004640824.1 Fe-S cluster assembly protein IscX -
  F2A31_RS02645 (F2A31_02645) ndk 548158..548589 (+) 432 WP_150025044.1 nucleoside-diphosphate kinase -
  F2A31_RS02650 (F2A31_02650) rlmN 548710..549945 (+) 1236 WP_150025045.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  F2A31_RS02655 (F2A31_02655) pilF 549963..550763 (+) 801 WP_026056343.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  F2A31_RS02660 (F2A31_02660) - 550754..551548 (+) 795 WP_004640817.1 helix-turn-helix domain-containing protein -
  F2A31_RS02665 (F2A31_02665) ispG 551570..552685 (+) 1116 WP_150025046.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  F2A31_RS02670 (F2A31_02670) hisS 552682..553974 (+) 1293 WP_150025047.1 histidine--tRNA ligase -
  F2A31_RS02675 (F2A31_02675) - 553999..554697 (+) 699 WP_150025048.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30220.21 Da        Isoelectric Point: 9.6050

>NTDB_id=386469 F2A31_RS02655 WP_026056343.1 549963..550763(+) (pilF) [Acinetobacter suaedae strain C16S1]
MNTTRLKTTIAITVVLMAGGLTACQSTPSTKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKLNSRDANANMMMGIL
LQQEGSKLNMEKADAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTRAGATLGYDQRFQSLENLGRIYLLLGDV
TNAEKSFKQALQANRNATISMLELSEIFYLQQNNRDASQLYEQYVRSVGQRNQGARALWIGIRIARANADQLGMQVLVNQ
LRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=386469 F2A31_RS02655 WP_026056343.1 549963..550763(+) (pilF) [Acinetobacter suaedae strain C16S1]
TTGAATACAACTCGACTAAAAACGACCATAGCGATAACAGTTGTACTTATGGCAGGTGGCTTGACAGCTTGCCAATCGAC
ACCATCTACGAAAGATCCAGAGAAAGCTGTTCAGGTTCGCACCCAATTGGCTGCGGAATATATTCGTACGCGTGATTTGG
ACTCTGCAAAACGTTCACTTGACCAAGCATTGAAACTTAATTCCCGTGATGCAAATGCCAATATGATGATGGGTATTTTG
CTGCAACAAGAGGGAAGTAAGCTCAATATGGAAAAAGCAGATGCTTATTTTAAGCGAGCGATTTCATCTGAGCCAGACAA
TGCTCAAGCGCGTAATAATTACGGTACATACTTATATCAGATGCAGCGTTATAATGAAGCAATTGAGCAGTTCACGCGTG
CTGGTGCAACCTTAGGTTATGATCAACGATTTCAATCACTGGAAAACCTAGGGCGAATCTATTTATTATTGGGCGATGTG
ACGAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCAAATCGAAATGCAACCATTTCAATGTTAGAGTTGTCGGAAAT
TTTCTATTTGCAACAAAACAATAGAGATGCATCCCAGTTATATGAACAATATGTTCGTAGCGTGGGACAGCGCAACCAAG
GTGCTAGAGCACTTTGGATCGGGATACGCATTGCTCGAGCGAATGCGGATCAGTTAGGAATGCAAGTGTTAGTCAACCAA
TTACGCGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.58

100

0.774
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment